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J. Biol. Chem., Vol. 260, Issue 16, 9114-9116, 08, 1985
Y Engstrom, S Eriksson, I Jildevik, S Skog, L Thelander and B Tribukait
Consistent with its specialized role in DNA synthesis, the activity of
ribonucleotide reductase is cell cycle-dependent, reaching its maximum
during S-phase. This paper demonstrates, however, the levels of the two
protein subunits, M1 and M2, of this enzyme vary independently of one
another. The level of protein M1 was determined by use of a two-site
monoclonal antibody-enzyme immunoassay and found to be constant throughout
the cell cycle in bovine kidney MDBK cells. Pulse-chase experiments showed
that the half-life of protein M1 was 15 h. This contrasts with our previous
results demonstrating an S-phase-correlated increase in the concentration
of protein M2 and a half-life of this subunit of 3 h. Therefore,
ribonucleotide reductase is controlled during the cell cycle by the level
of protein M2.
Cell cycle-dependent expression of mammalian ribonucleotide reductase. Differential regulation of the two subunits
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