HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foreman, J. E. Articles by Niehaus, W. G. Search for Related Content PubMed PubMed Citation Articles by Foreman, J. E. Articles by Niehaus, W. G., Jr Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 18, 10019-10022, Aug, 1985

Zn2+-induced cooperativity of mannitol-1-phosphate dehydrogenase from Aspergillus parasiticus

JE Foreman and WG Niehaus Jr

Mannitol-1-phosphate dehydrogenase (EC 1.1.1.17) has been purified from Aspergillus parasiticus, a filamentous fungus which produces the polyketide mycotoxin, versicolorin A. Its kinetic properties have been compared with those of mannitol-1-phosphate dehydrogenase from the related non-toxin-producing fungus, A. niger. Both enzymes are inhibited by divalent transition metals, especially Zn2+ and Cd2+, but only the enzyme from A. parasiticus exhibits inhibitor-induced cooperative binding of the substrate, fructose-6 phosphate. Double reciprocal plots (1/v versus 1/Fru-6-P) are linear in the absence of Zn2+ but in the presence of Zn2+ are concave upward, with Hill coefficients of 1.5. The extent of cooperativity is inversely related to ionic strength, disappearing at 100 mM KCl. The enzymes from both organisms are relatively stable to incubation at 30 degrees C, but only the enzyme from A. parasiticus is rendered thermally unstable by the addition of divalent transition metals. A model is proposed to explain how binding of transition metal ions affects substrate binding and thermal stability of the enzyme.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Iwamoto, H. Kawanobe, T. Ikawa, and Y. Shiraiwa Characterization of Salt-Regulated Mannitol-1-Phosphate Dehydrogenase in the Red Alga Caloglossa continua Plant Physiology, October 1, 2003; 133(2): 893 - 900. [Abstract] [Full Text] [PDF]

				K. Suvarna, A. Bartiss, and B. Wong Mannitol-1-phosphate dehydrogenase from Cryptococcus neoformans is a zinc-containing long-chain alcohol/polyol dehydrogenase Microbiology, October 1, 2000; 146(10): 2705 - 2713. [Abstract] [Full Text]

				M. Caligiuri and R Bauerle Subunit communication in the anthranilate synthase complex from Salmonella typhimurium Science, June 28, 1991; 252(5014): 1845 - 1848. [Abstract] [PDF]

				L. Kuo, I Zambidis, and C Caron Triggering of allostery in an enzyme by a point mutation: ornithine transcarbamoylase Science, August 4, 1989; 245(4917): 522 - 524. [Abstract] [PDF]