| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 260, Issue 18, 10081-10086, Aug, 1985
SJ Pandol, MS Schoeffield, G Sachs and S Muallem
To determine the role of free cytosolic calcium ([Ca+2]i) in stimulated
enzyme secretion from exocrine pancreas, we determined the effects of
various pancreatic secretagogues on [Ca+2]i and amylase release in
dispersed acini from the guinea pig pancreas. Cholecystokinin- octapeptide
(CCK-OP), carbachol, and bombesin, but not vasoactive intestinal peptide,
stimulated rapid increases in [Ca+2]i from 100 to 600-800 nM that were
independent of extracellular calcium. The increases in [Ca+2]i were
transient (lasting less than 5 min) and correlated with an initial rapid
phase of amylase release. After 5 min, secretagogue-stimulated amylase
release occurred at basal [Ca+2]i. Carbachol pretreatment of the acini
abolished the effects of CCK-OP and bombesin on [Ca+2]i and the initial
rapid phase of amylase release. 4 beta-phorbol 12-myristate 13-acetate
(PMA) had no effect on [Ca+2]i but stimulated an increase in amylase
release. The addition of CCK-OP or A23187 to PMA-stimulated acini caused an
increase in [Ca+2]i and PMA- stimulated amylase release only during the
first 5 min after addition of these agents. These results indicate that
CCK-OP, carbachol, and bombesin release calcium from an intracellular pool,
resulting in a transient increase in [Ca+2]i and that this increase in
[Ca+2]i mediates enzyme secretion during the first few minutes of
incubation. The results with PMA suggest that secretagogue-stimulated
secretion not mediated by increased [Ca+2]i (sustained secretion) is
mediated by 1,2- diacylglycerol.
Role of free cytosolic calcium in secretagogue-stimulated amylase release from dispersed acini from guinea pig pancreas
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Hirohata, M. Fujii, Y. Okabayashi, Y. Nagashio, M. Tashiro, I. Imoto, T. Akiyama, and M. Otsuki Stimulatory effects of bilirubin on amylase release from isolated rat pancreatic acini Am J Physiol Gastrointest Liver Physiol, February 1, 2002; 282(2): G249 - G256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Poosti, L. di Malta, D. Gagne, N. Bernad, J.-C. Galleyrand, C. Escrieut, S. Silvente-Poirot, D. Fourmy, and J. Martinez The Third Intracellular Loop of the Rat and Mouse Cholecystokinin-A Receptors Is Responsible for Different Patterns of Gene Activation Mol. Pharmacol., April 13, 2001; 58(6): 1381 - 1388. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Noble, S. A. Wank, J. N. Crawley, J. Bradwejn, K. B. Seroogy, M. Hamon, and B. P. Roques International Union of Pharmacology. XXI. Structure, Distribution, and Functions of Cholecystokinin Receptors Pharmacol. Rev., December 1, 1999; 51(4): 745 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Feick, S. Gilhaus, and I. Schulz Pervanadate Stimulates Amylase Release and Protein Tyrosine Phosphorylation of Paxillin and p125FAK in Differentiated AR4-2J Pancreatic Acinar Cells J. Biol. Chem., June 26, 1998; 273(26): 16366 - 16373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Jena, S. W. Schneider, J. P. Geibel, P. Webster, H. Oberleithner, and K. C. Sritharan Gi regulation of secretory vesicle swelling examined by atomic force microscopy PNAS, November 25, 1997; 94(24): 13317 - 13322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xu, W. Zeng, J. Diaz, and S. Muallem Spacial Compartmentalization of Ca2+ Signaling Complexes in Pancreatic Acini J. Biol. Chem., October 4, 1996; 271(40): 24684 - 24690. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
