| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 260, Issue 27, 14571-14579, 11, 1985
SE Saucier, AA Kandutsch, FR Taylor, TA Spencer, S Phirwa and AK Gayen
Biosynthetically tritiated sterols from Chinese hamster lung (Dede) cells
were fractionated by high performance liquid chromatography, and fractions
were assayed for their ability to repress 3-hydroxy-3- methylglutaryl-CoA
reductase in L cell cultures. Most of the activity found was associated
with two oxysterols, 24(S),25-epoxycholesterol and 25-hydroxycholesterol.
The identities of the two sterols were established by co-chromatography
with authentic samples and by isotopic dilution and recrystallization. Only
low levels of repressor activity were found in other fractions of the
sterol extract. The endogenous concentrations of 24(S),25-epoxycholesterol
(7.2 fg/cell) and 25- hydroxycholesterol (1.5 fg/cell) appear to be within
the ranges required for the regulation of HMG-CoA reductase.
Identification of regulatory oxysterols, 24(S),25-epoxycholesterol and 25-hydroxycholesterol, in cultured fibroblasts
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Wong, C. M. Quinn, I. C. Gelissen, and A. J. Brown Endogenous 24(S),25-Epoxycholesterol Fine-tunes Acute Control of Cellular Cholesterol Homeostasis J. Biol. Chem., January 11, 2008; 283(2): 700 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, W. M. Pandak, S. K. Erickson, Y. Ma, L. Yin, P. Hylemon, and S. Ren Biosynthesis of the regulatory oxysterol, 5-cholesten-3{beta},25-diol 3-sulfate, in hepatocytes J. Lipid Res., December 1, 2007; 48(12): 2587 - 2596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ren, P. Hylemon, Z.-P. Zhang, D. Rodriguez-Agudo, D. Marques, X. Li, H. Zhou, G. Gil, and W. M. Pandak Identification of a novel sulfonated oxysterol, 5-cholesten-3{beta},25-diol 3-sulfonate, in hepatocyte nuclei and mitochondria J. Lipid Res., May 1, 2006; 47(5): 1081 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kuge and M. Nishijima Biosynthetic Regulation and Intracellular Transport of Phosphatidylserine in Mammalian Cells J. Biochem., April 1, 2003; 133(4): 397 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, D. Li, D. E. Blanchard, S. R. Lear, S. K. Erickson, and T. A. Spencer Key regulatory oxysterols in liver: analysis as {{Delta}}4-3-ketone derivatives by HPLC and response to physiological perturbations J. Lipid Res., April 1, 2001; 42(4): 649 - 658. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Song and S. Liao Cholestenoic Acid Is a Naturally Occurring Ligand for Liver X Receptor {alpha} Endocrinology, November 1, 2000; 141(11): 4180 - 4184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Schroepfer Jr. Oxysterols: Modulators of Cholesterol Metabolism and Other Processes Physiol Rev, January 1, 2000; 80(1): 361 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Lala, P. M. Syka, S. B. Lazarchik, D. J. Mangelsdorf, K. L. Parker, and R. A. Heyman Activation of the orphan nuclear receptor steroidogenic factor 1 by oxysterols PNAS, May 13, 1997; 94(10): 4895 - 4900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Lehmann, S. A. Kliewer, L. B. Moore, T. A. Smith-Oliver, B. B. Oliver, J.-L. Su, S. S. Sundseth, D. A. Winegar, D. E. Blanchard, T. A. Spencer, et al. Activation of the Nuclear Receptor LXR by Oxysterols Defines a New Hormone Response Pathway J. Biol. Chem., February 7, 1997; 272(6): 3137 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sakakibara, R. Watanabe, Y. Kanai, and T. Ono Molecular Cloning and Expression of Rat Squalene Epoxidase J. Biol. Chem., January 6, 1995; 270(1): 17 - 20. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
