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J. Biol. Chem., Vol. 260, Issue 29, 15510-15515, 12, 1985
DK Podolsky
Purified human colonic mucin contains six distinct components which may be
separated by DEAE-cellulose chromatography. Past studies defined the
structure of oligosaccharide side chains from the most abundant species
III, IV, and V which elute at intermediate salt concentrations. In these
studies the structures of oligosaccharide side chains liberated from the
remaining early and late eluting species I, II, and VI were determined
after isolation by sequential conventional and high performance liquid
chromatography through combination of gas chromatography, methylation
analysis, and sequential glycosidase digestion. Mucin species I, II, and VI
contained a less varied array of discrete oligosaccharide structures than
that observed in the major mucin components. Mucin species I and II
contained five and 10 structures, respectively, which account for 68 and
71% of total oligosaccharide content in these fractions. The predominant
oligosaccharides of mucin species I included three neutral structures: a
disaccharide GlcNAc beta (1-3)GalNAc-ol, a trisaccharide Gal beta (1-
4)GlcNAc beta (1-3)GalNAc-ol, and a tetrasaccharide GlcNAc beta (1- 4)Gal
beta (1-4)GlcNAc beta (1-3)GalNAc-ol as well as two acidic components
representing the sialylated forms of two of these oligosaccharides. Mucin
species II contained these same oligosaccharides as well as four additional
acidic structures, notably a disaccharide Neu alpha (2-6)GalNAc-ol and a
hexasaccharide Gal beta (1-4)GlcNAc beta (1-3)Gal beta (1-4)GlcNAc beta
(1-3) (NeuAc alpha (2- 6))-GalNAc-ol, not identified in any other mucin
species. The late eluting mucin species VI contained at least five discrete
neutral oligosaccharides and six major acidic structures. While the
majority of these structures had been previously isolated from the earlier
eluting mucin species IV and V, species VI also contained di- and
trisialylated oligosaccharides not identified in other mucin species. In
conjunction with earlier studies of the major mucin species III, IV, and V,
these data define the range of oligosaccharide structures present in human
colonic mucin. These studies demonstrate that human colonic mucin possesses
species with characteristic and distinguishable combinations of
oligosaccharides which reflect variations of common core structures.
Oligosaccharide structures of isolated human colonic mucin species
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