HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shahak, Y. Search for Related Content PubMed PubMed Citation Articles by Shahak, Y. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 3, 1459-1464, 02, 1985

Differential effect of thiol oxidants on the chloroplast H+-ATPase in the light and in the dark

Y Shahak

The effect of thiol oxidants on the light-activated H+-ATPase has been studied in freshly broken and intact chloroplasts. The following observations were made: (i) in chloroplasts which are osmotically shocked after light activation, ferricyanide stimulates the deactivation of the enzyme in the dark, but has little effect in the light; (ii) similarly, o-iodosobenzoate is a most efficient deactivator of the ATPase in intact chloroplasts in the dark but not in the light; (iii) the activated ATPase becomes sensitive to oxidants in the light upon the addition of an uncoupler; (iv) the oxidant-induced deactivation in the dark dominates the stabilizing effect of pyrophosphate or ADP plus Mg2+; (v) full deactivation of the ATPase by dark adaptation or by oxidants does not affect the rate of photophosphorylation under saturating conditions. A model is suggested in which two kinds of conformational changes are involved in the regulation of the ATPase: those induced by the trans-membrane-proton gradient and those by oxidation-reduction of the enzyme. These changes result in the preferential interaction with thiol reductants in the light but with thiol oxidants in the dark.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Rintamäki, P. Martinsuo, S. Pursiheimo, and E.-M. Aro Cooperative regulation of light-harvesting complex II phosphorylation via the plastoquinol and ferredoxin-thioredoxin system in chloroplasts PNAS, September 22, 2000; (2000) 180054297. [Abstract] [Full Text]

				S. A. Ross, M. X. Zhang, and B. R. Selman Role of the Chlamydomonas reinhardtii Coupling Factor 1 [IMAGE]-Subunit Cysteine Bridge in the Regulation of ATP Synthase J. Biol. Chem., April 28, 1995; 270(17): 9813 - 9818. [Abstract] [Full Text] [PDF]

				E. Rintamaki, P. Martinsuo, S. Pursiheimo, and E.-M. Aro Cooperative regulation of light-harvesting complex II phosphorylation via the plastoquinol and ferredoxin-thioredoxin system in chloroplasts PNAS, October 10, 2000; 97(21): 11644 - 11649. [Abstract] [Full Text] [PDF]