| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 260, Issue 30, 16250-16254, 12, 1985
A Jacobsson, U Stadler, MA Glotzer and LP Kozak
We have identified cDNAs clones for several cold-inducible mRNAs from the
brown adipose tissue of mice. pCIN-1, a plasmid with a 900-base pair
insert, encoded the mitochondrial uncoupling protein (UCP) as determined by
the ability of the cDNA insert to select, by hybridization, an mRNA that
could be translated into a 32,000-Da protein immunoprecipitable with
anti-UCP antibodies. Nine tissues were analyzed; however, UCP cDNA
hybridized to an mRNA species of 1.6 and 2.0 kilobase pairs only in brown
adipose tissue. A maximum induction of 10-fold occurred within 6 h of
exposure to cold (5 degrees C). A BamHI restriction fragment polymorphism
detected by Southern blot analysis of genomic DNA in recombinant inbred
mouse strains allowed us to map the UCP gene to Chromosome 8. The analysis
of the UCP gene expression in diabetic (db) and obese (ob) mice maintained
at 27 degrees C for 3 days followed by cold exposure for 4 h at 5 degrees C
indicated that UCP mRNA levels in mutant mice were unaffected at 27 degrees
C and only slightly reduced at 5 degrees C. Accordingly, the inability of
diabetic and obese mice to thermoregulate is not associated with a lack of
UCP mRNA induction.
Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Endo and T. Kobayashi Thyroid-stimulating hormone receptor in brown adipose tissue is involved in the regulation of thermogenesis Am J Physiol Endocrinol Metab, August 1, 2008; 295(2): E514 - E518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. V. Kramarova, I. G. Shabalina, U. Andersson, R. Westerberg, I. Carlberg, J. Houstek, J. Nedergaard, and B. Cannon Mitochondrial ATP synthase levels in brown adipose tissue are governed by the c-Fo subunit P1 isoform FASEB J, January 1, 2008; 22(1): 55 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Frontini, S. Rousset, A.-M. Cassard-Doulcier, C. Zingaretti, D. Ricquier, and S. Cinti Thymus Uncoupling Protein 1 Is Exclusive to Typical Brown Adipocytes and Is Not Found in Thymocytes J. Histochem. Cytochem., February 1, 2007; 55(2): 183 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Anzulovich, A. Mir, M. Brewer, G. Ferreyra, C. Vinson, and R. Baler Elovl3: a model gene to dissect homeostatic links between the circadian clock and nutritional status J. Lipid Res., December 1, 2006; 47(12): 2690 - 2700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li, K. Prabhakaran, X. Zhang, J. L. Borowitz, and G. E. Isom PPAR{alpha}-Mediated Upregulation of Uncoupling Protein-2 Switches Cyanide-Induced Apoptosis to Necrosis in Primary Cortical Cells Toxicol. Sci., September 1, 2006; 93(1): 136 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yoshinari, N. Okino, T. Sato, J. Sugatani, and M. Miwa INDUCTION OF DETOXIFYING ENZYMES IN RODENT WHITE ADIPOSE TISSUE BY ARYL HYDROCARBON RECEPTOR AGONISTS AND ANTIOXIDANTS Drug Metab. Dispos., July 1, 2006; 34(7): 1081 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ukropec, R. V. P. Anunciado, Y. Ravussin, and L. P. Kozak Leptin Is Required for Uncoupling Protein-1-Independent Thermogenesis during Cold Stress Endocrinology, May 1, 2006; 147(5): 2468 - 2480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Westerberg, J.-E. Mansson, V. Golozoubova, I. G. Shabalina, E. C. Backlund, P. Tvrdik, K. Retterstol, M. R. Capecchi, and A. Jacobsson ELOVL3 Is an Important Component for Early Onset of Lipid Recruitment in Brown Adipose Tissue J. Biol. Chem., February 24, 2006; 281(8): 4958 - 4968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Xue, A. Coulter, J. S. Rim, R. A. Koza, and L. P. Kozak Transcriptional Synergy and the Regulation of Ucp1 during Brown Adipocyte Induction in White Fat Depots Mol. Cell. Biol., September 15, 2005; 25(18): 8311 - 8322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Reifel-Miller, K. Otto, E. Hawkins, R. Barr, W. R. Bensch, C. Bull, S. Dana, K. Klausing, J.-A. Martin, R. Rafaeloff-Phail, et al. A Peroxisome Proliferator-Activated Receptor {alpha}/{gamma} Dual Agonist with a Unique in Vitro Profile and Potent Glucose and Lipid Effects in Rodent Models of Type 2 Diabetes and Dyslipidemia Mol. Endocrinol., June 1, 2005; 19(6): 1593 - 1605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Nolan, M. A. Sikorski, and G. S. McKnight The Role of Uncoupling Protein 1 in the Metabolism and Adiposity of RII{beta}-Protein Kinase A-Deficient Mice Mol. Endocrinol., September 1, 2004; 18(9): 2302 - 2311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Chiu, W.-D. Lin, S.-Y. Huang, and Y.-H. Lee Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells Genes & Dev., August 15, 2004; 18(16): 1970 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Rim, B. Xue, B. Gawronska-Kozak, and L. P. Kozak Sequestration of Thermogenic Transcription Factors in the Cytoplasm during Development of Brown Adipose Tissue J. Biol. Chem., June 11, 2004; 279(24): 25916 - 25926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xiao, D. Massaro, G. D. Massaro, and L. B. Clerch Expression of Lung Uncoupling Protein-2 mRNA Is Modulated Developmentally and by Caloric Intake Experimental Biology and Medicine, June 1, 2004; 229(6): 479 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Cao, K. W. Daniel, J. Robidoux, P. Puigserver, A. V. Medvedev, X. Bai, L. M. Floering, B. M. Spiegelman, and S. Collins p38 Mitogen-Activated Protein Kinase Is the Central Regulator of Cyclic AMP-Dependent Transcription of the Brown Fat Uncoupling Protein 1 Gene Mol. Cell. Biol., April 1, 2004; 24(7): 3057 - 3067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Golozoubova, H. Gullberg, A. Matthias, B. Cannon, B. Vennstrom, and J. Nedergaard Depressed Thermogenesis but Competent Brown Adipose Tissue Recruitment in Mice Devoid of All Hormone-Binding Thyroid Hormone Receptors Mol. Endocrinol., February 1, 2004; 18(2): 384 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. CANNON and J. NEDERGAARD Brown Adipose Tissue: Function and Physiological Significance Physiol Rev, January 1, 2004; 84(1): 277 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Hansen, S. M. Krasnow, M. A. Nolan, G. S. Fraley, J. W. Baumgartner, D. K. Clifton, and R. A. Steiner Activation of the Sympathetic Nervous System by Galanin-Like Peptide--A Possible Link between Leptin and Metabolism Endocrinology, November 1, 2003; 144(11): 4709 - 4717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Rodriguez, P. Roca, M. L. Bonet, C. Pico, P. Oliver, and A. Palou Positive correlation of skeletal muscle UCP3 mRNA levels with overweight in male, but not in female, rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R880 - R888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Horton, I. Shimomura, S. Ikemoto, Y. Bashmakov, and R. E. Hammer Overexpression of Sterol Regulatory Element-binding Protein-1a in Mouse Adipose Tissue Produces Adipocyte Hypertrophy, Increased Fatty Acid Secretion, and Fatty Liver J. Biol. Chem., September 19, 2003; 278(38): 36652 - 36660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Smith Richards, B. N. Belton, A. C. Poole, J. J. Mancuso, G. A. Churchill, R. Li, J. Volaufova, A. Zuberi, and B. York QTL analysis of self-selected macronutrient diet intake: fat, carbohydrate, and total kilocalories Physiol Genomics, December 3, 2002; 11(3): 205 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Shabalina, C. Wiklund, T. Bengtsson, A. Jacobsson, B. Cannon, and J. Nedergaard Uncoupling protein-1: involvement in a novel pathway for beta -adrenergic, cAMP-mediated intestinal relaxation Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1107 - G1116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Rim and L. P. Kozak Regulatory Motifs for CREB-binding Protein and Nfe2l2 Transcription Factors in the Upstream Enhancer of the Mitochondrial Uncoupling Protein 1 Gene J. Biol. Chem., September 6, 2002; 277(37): 34589 - 34600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hausberg, D. A. Morgan, J. L. Mitchell, W. I. Sivitz, A. L. Mark, and W. G. Haynes Leptin Potentiates Thermogenic Sympathetic Responses to Hypothermia: A Receptor-Mediated Effect Diabetes, August 1, 2002; 51(8): 2434 - 2440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rodriguez, J. Ribot, and A. Palou trans-10, cis-12, but not cis-9, trans-11 CLA isomer, inhibits brown adipocyte thermogenic capacity Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1789 - R1797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nibbelink, K. Moulin, E. Arnaud, C. Duval, L. Penicaud, and L. Casteilla Brown Fat UCP1 Is Specifically Expressed in Uterine Longitudinal Smooth Muscle Cells J. Biol. Chem., December 7, 2001; 276(50): 47291 - 47295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ste. Marie, G. I. Miura, D. J. Marsh, K. Yagaloff, and R. D. Palmiter A metabolic defect promotes obesity in mice lacking melanocortin-4 receptors PNAS, October 4, 2000; (2000) 220409497. [Abstract] [Full Text]
|
|
|
|
|
|
A. Matthias, A. Jacobsson, B. Cannon, and J. Nedergaard The Bioenergetics of Brown Fat Mitochondria from UCP1-ablated Mice. UCP1 IS NOT INVOLVED IN FATTY ACID-INDUCED DE-ENERGIZATION ("UNCOUPLING") J. Biol. Chem., October 1, 1999; 274(40): 28150 - 28160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Guardiola-Diaz, S. Rehnmark, N. Usuda, T. Albrektsen, D. Feltkamp, J.-A. Gustafsson, and S. E. H. Alexson Rat Peroxisome Proliferator-activated Receptors and Brown Adipose Tissue Function during Cold Acclimatization J. Biol. Chem., August 13, 1999; 274(33): 23368 - 23377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Li, J. O. Holloszy, and C. F. Semenkovich Respiratory Uncoupling Induces delta -Aminolevulinate Synthase Expression through a Nuclear Respiratory Factor-1-dependent Mechanism in HeLa Cells J. Biol. Chem., June 18, 1999; 274(25): 17534 - 17540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Commins, P. M. Watson, M. A. Padgett, A. Dudley, G. Argyropoulos, and T. W. Gettys Induction of Uncoupling Protein Expression in Brown and White Adipose Tissue by Leptin Endocrinology, January 1, 1999; 140(1): 292 - 300. [Abstract] [Full Text]
|
|
|
|
|
|
M. Branco, M. Ribeiro, N. Negrao, and A. C. Bianco 3,5,3'-Triiodothyronine actively stimulates UCP in brown fat under minimal sympathetic activity Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E179 - E187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kushi, H. Sasai, H. Koizumi, N. Takeda, M. Yokoyama, and M. Nakamura Obesity and mild hyperinsulinemia found in neuropeptide Y-Y1 receptor-deficient mice PNAS, December 22, 1998; 95(26): 15659 - 15664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Moitra, M. M. Mason, M. Olive, D. Krylov, O. Gavrilova, B. Marcus-Samuels, L. Feigenbaum, E. Lee, T. Aoyama, M. Eckhaus, et al. Life without white fat: a transgenic mouse Genes & Dev., October 15, 1998; 12(20): 3168 - 3181. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Surwit, S. Wang, A. E. Petro, D. Sanchis, S. Raimbault, D. Ricquier, and S. Collins Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice PNAS, March 31, 1998; 95(7): 4061 - 4065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Tvrdik, A. Asadi, L. P. Kozak, J. Nedergaard, B. Cannon, and A. Jacobsson Cig30, a Mouse Member of a Novel Membrane Protein Gene Family, Is Involved in the Recruitment of Brown Adipose Tissue J. Biol. Chem., December 12, 1997; 272(50): 31738 - 31746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Solanes, A. Vidal-Puig, D. Grujic, J. S. Flier, and B. B. Lowell The Human Uncoupling Protein-3 Gene. GENOMIC STRUCTURE, CHROMOSOMAL LOCALIZATION, AND GENETIC BASIS FOR SHORT AND LONG FORM TRANSCRIPTS J. Biol. Chem., October 10, 1997; 272(41): 25433 - 25436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-W. Gong, Y. He, M. Karas, and M. Reitman Uncoupling Protein-3 Is a Mediator of Thermogenesis Regulated by Thyroid Hormone, beta 3-Adrenergic Agonists, and Leptin J. Biol. Chem., September 26, 1997; 272(39): 24129 - 24132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Larose, A.-M. Cassard-Doulcier, C. Fleury, F. Serra, O. Champigny, F. Bouillaud, and D. Ricquier Essential cis-Acting Elements in Rat Uncoupling Protein Gene Are in an Enhancer Containing a Complex Retinoic Acid Response Domain J. Biol. Chem., December 6, 1996; 271(49): 31533 - 31542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Houstek, U. Andersson, P. Tvrdík, J. Nedergaard, and B. Cannon The Expression of Subunit c Correlates with and Thus May Limit the Biosynthesis of the Mitochondrial F(0)F(1)-ATPase in Brown Adipose Tissue J. Biol. Chem., March 31, 1995; 270(13): 7689 - 7694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Alvarez, J. de Andrés, P. Yubero, O. Vińas, T. Mampel, R. Iglesias, M. Giralt, and F. Villarroya A Novel Regulatory Pathway of Brown Fat Thermogenesis J. Biol. Chem., March 10, 1995; 270(10): 5666 - 5673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J D Saffer, S P Jackson, and S J Thurston SV40 stimulates expression of the transacting factor Sp1 at the mRNA level. Genes & Dev., April 1, 1990; 4(4): 659 - 666. [Abstract] [PDF]
|
|
|
|
|
|
E H Birkenmeier, B Gwynn, S Howard, J Jerry, J I Gordon, W H Landschulz, and S L McKnight Tissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein. Genes & Dev., August 1, 1989; 3(8): 1146 - 1156. [Abstract] [PDF]
|
|
|
|
|
|
M. del Mar Gonzalez-Barroso, C. Pecqueur, C. Gelly, D. Sanchis, M.-C. Alves-Guerra, F. Bouillaud, D. Ricquier, and A.-M. Cassard-Doulcier Transcriptional Activation of the Human ucp1 Gene in a Rodent Cell Line. SYNERGISM OF RETINOIDS, ISOPROTERENOL, AND THIAZOLIDINEDIONE IS MEDIATED BY A MULTIPARTITE RESPONSE ELEMENT J. Biol. Chem., October 6, 2000; 275(41): 31722 - 31732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Huppertz, B. M. Fischer, Y.-B. Kim, K. Kotani, A. Vidal-Puig, L. J. Slieker, K. W. Sloop, B. B. Lowell, and B. B. Kahn Uncoupling Protein 3 (UCP3) Stimulates Glucose Uptake in Muscle Cells through a Phosphoinositide 3-Kinase-dependent Mechanism J. Biol. Chem., April 13, 2001; 276(16): 12520 - 12529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. E. Hofmann, X. Liu, C. M. Bearden, M.-E. Harper, and L. P. Kozak Effects of Genetic Background on Thermoregulation and Fatty Acid-induced Uncoupling of Mitochondria in UCP1-deficient Mice J. Biol. Chem., April 6, 2001; 276(15): 12460 - 12465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Olswang, H. Cohen, O. Papo, H. Cassuto, C. M. Croniger, P. Hakimi, S. M. Tilghman, R. W. Hanson, and L. Reshef A mutation in the peroxisome proliferator-activated receptor gamma -binding site in the gene for the cytosolic form of phosphoenolpyruvate carboxykinase reduces adipose tissue size and fat content in mice PNAS, January 22, 2002; 99(2): 625 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ste. Marie, G. I. Miura, D. J. Marsh, K. Yagaloff, and R. D. Palmiter A metabolic defect promotes obesity in mice lacking melanocortin-4 receptors PNAS, October 24, 2000; 97(22): 12339 - 12344. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
