HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van Berkel, T. J. Articles by Kempen, H. J. Search for Related Content PubMed PubMed Citation Articles by van Berkel, T. J. Articles by Kempen, H. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 5, 2694-2699, 03, 1985

The effect of a water-soluble tris-galactoside-terminated cholesterol derivative on the fate of low density lipoproteins and liposomes

TJ van Berkel, JK Kruijt, HH Spanjer, JF Nagelkerke, L Harkes and HJ Kempen

A triantennary galactose-terminated cholesterol derivative, N- (tris(beta-D-galactopyranosyloxymethyl) methyl)-N alpha-(4-(5-cholesten- 3 beta-yloxy)succinyl)glycinamide (Tris-Gal-Chol), which dissolves easily in water, was added to human low density lipoproteins (LDL) in varying quantities. Upon addition to LDL, Tris-Gal-Chol was immediately incorporated, and after intravenous injection into rats, the iodine- labeled apolipoprotein B radioactivity was readily associated with the liver. The incorporation of 5 or 13 micrograms of Tris-Gal-Chol into LDL (20 micrograms of protein) stimulates the parenchymal cell association of LDL 6- and 10-fold, respectively, at 10 min after injection. For non-parenchymal cells, the cell association is 60- and 70-fold stimulated, respectively. It can be calculated that non- parenchymal cells (mainly Kupffer cells) are for 80-90% responsible for the increased, galactose-mediated, interaction of Tris-Gal-Chol LDL with the liver. The increased interaction of LDL with the cells upon Tris-Gal-Chol incorporation is followed by degradation of the apolipoprotein B in the lysosomes. Incorporation of Tris-Gal-Chol into unilamellar liposomes (10 mol %) leads to an increased cell association of the enclosed [3H]inulin to parenchymal cells (1.4-fold) and non- parenchymal cells (11.8-fold). It is concluded that Tris-Gal-Chol incorporation into LDL leads to a markedly increased catabolism of LDL by the liver which might be used for lowering serum LDL levels. The possibility of increasing the interaction of LDL or liposomes with specific liver cell types by Tris-Gal-Chol might also have an application for targeting drugs or other compounds of interest to these cells.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. C.N. Rensen, L. A.J.M. Sliedregt, P. J. van Santbrink, M. Ferns, H. N.J. Schifferstein, S. H. van Leeuwen, J. H.M. Souverijn, T. J.C. van Berkel, and E. A.L. Biessen Stimulation of Liver-Directed Cholesterol Flux in Mice by Novel N-Acetylgalactosamine-Terminated Glycolipids With High Affinity for the Asialoglycoprotein Receptor Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 169 - 175. [Abstract] [Full Text] [PDF]

				G. Baillie, M. D. Owens, and G. W. Halbert A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro J. Lipid Res., January 1, 2002; 43(1): 69 - 73. [Abstract] [Full Text] [PDF]

				V. Terpstra and T. J. C. van Berkel Scavenger receptors on liver Kupffer cells mediate the in vivo uptake of oxidatively damaged red blood cells in mice Blood, March 15, 2000; 95(6): 2157 - 2163. [Abstract] [Full Text] [PDF]

				E. A. L. Biessen, H. Vietsch, J. Kuiper, M. K. Bijsterbosch, and T. J. C. v. Berkel Liver Uptake of Phosphodiester Oligodeoxynucleotides Is Mediated by Scavenger Receptors Mol. Pharmacol., February 1, 1998; 53(2): 262 - 269. [Abstract] [Full Text]

				E. A.L. Biessen, H. Vietsch, and T. J.C. van Berkel Induction of Hepatic Uptake of Lipoprotein(a) by Cholesterol-Derivatized Cluster Galactosides Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1552 - 1558. [Abstract] [Full Text]

				E. A.L. Biessen, H. Vietsch, and T. J.C. Van Berkel Cholesterol Derivative of a New Triantennary Cluster Galactoside Lowers Serum Cholesterol Levels and Enhances Secretion of Bile Acids in the Rat Circulation, March 15, 1995; 91(6): 1847 - 1854. [Abstract] [Full Text]

				P. C. N. Rensen, L. A. J. M. Sliedregt, M. Ferns, E. Kieviet, S. M. W. van Rossenberg, S. H. van Leeuwen, T. J. C. van Berkel, and E. A. L. Biessen Determination of the Upper Size Limit for Uptake and Processing of Ligands by the Asialoglycoprotein Receptor on Hepatocytes in Vitro and in Vivo J. Biol. Chem., September 28, 2001; 276(40): 37577 - 37584. [Abstract] [Full Text] [PDF]