HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonne, D. Articles by Pantaloni, D. Search for Related Content PubMed PubMed Citation Articles by Bonne, D. Articles by Pantaloni, D. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 5, 2819-2825, 03, 1985

4',6-Diamidino-2-phenylindole, a fluorescent probe for tubulin and microtubules

D Bonne, C Heusele, C Simon and D Pantaloni

A new fluorophor for tubulin which has permitted the monitoring of microtubule assembly in vitro is reported. DAPI (4',6-diamidino-2- phenylindole), a fluorophor already known as a DNA intercalator, was shown to bind specifically to a unique tubulin site as a dimer (KD(app) = 43 +/- 5 microM at 37 degrees C) or to tubulin associated in microtubules (KD(app) = 6 +/- 2 microM at 37 degrees C) with the same maximum enhancement in fluorescence. When tubulin polymerization was induced with GTP, the change in DAPI affinity for tubulin resulted in an enhancement of DAPI binding and, consequently, of fluorescence intensity. DAPI, whose binding site is different from that of colchicine, vinblastine, or taxol, did not interfere greatly with microtubule polymerization. It induced a slight diminution of the critical concentration for tubulin assembly due to a decrease in the depolymerizing rate constant. Moreover, DAPI did not interfere with GTP hydrolysis correlated with tubulin polymerization, but it decreased the GTPase activity at the steady state of tubulin assembly. Even at substoichiometric levels DAPI can be used to follow the kinetics of microtubule assembly.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. L. Gururaja, D. Goff, T. Kinoshita, E. Goldstein, S. Yung, J. McLaughlin, E. Pali, J. Huang, R. Singh, S. Daniel-Issakani, et al. R-253 disrupts microtubule networks in multiple tumor cell lines. Clin. Cancer Res., June 15, 2006; 12(12): 3831 - 3842. [Abstract] [Full Text] [PDF]

				E. G. Chedrawy, J.-S. Wang, D. M. Nguyen, D. Shum-Tim, and R. C. J. Chiu Incorporation and integration of implanted myogenic and stem cells into native myocardial fibers: Anatomic basis for functional improvements J. Thorac. Cardiovasc. Surg., September 1, 2002; 124(3): 584 - 590. [Abstract] [Full Text] [PDF]

				J.-S. Wang, D. Shum-Tim, J. Galipeau, E. Chedrawy, N. Eliopoulos, and R. C.-J. Chiu Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages J. Thorac. Cardiovasc. Surg., November 1, 2000; 120(5): 999 - 1006. [Abstract] [Full Text] [PDF]

				C. Papaseit, N. Pochon, and J. Tabony Microtubule self-organization is gravity-dependent PNAS, June 30, 2000; (2000) 140029597. [Abstract] [Full Text]

				I Lajoie-Mazenc, Y Tollon, C Detraves, M Julian, A Moisand, C Gueth-Hallonet, A Debec, I Salles-Passador, A Puget, H Mazarguil, et al. Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindle J. Cell Sci., January 10, 1994; 107(10): 2825 - 2837. [Abstract] [PDF]

				C. Papaseit, N. Pochon, and J. Tabony Microtubule self-organization is gravity-dependent PNAS, July 18, 2000; 97(15): 8364 - 8368. [Abstract] [Full Text] [PDF]