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J. Biol. Chem., Vol. 260, Issue 6, 3247-3250, 03, 1985
CJ Omiecinski, FG Walz Jr and GP Vlasuk
Cytochromes P-450b and P-450e are extremely homologous and immunochemically
indistinguishable proteins that are coordinately induced by phenobarbital
in rat liver. To assess the effect of phenobarbital on mRNA levels for each
of these hemoproteins we performed solution hybridization and Northern blot
experiments with synthetic oligodeoxynucleotide probes of defined sequence.
Our data demonstrate that phenobarbital administration to rats resulted in
marked increases in levels of hepatic mRNA for both cytochrome P-450b and
cytochrome P-450e, with a 4- to 5-fold greater accumulation of P- 450b mRNA
vis a vis P-450e mRNA. The level of hepatic mRNA increased from less than 3
molecules/cell of each mRNA in untreated rats, to 630 and 130
molecules/cell for P-450b and P-450e, respectively, in
phenobarbital-treated rats. Data obtained in Northern blot hybridization
experiments demonstrated that the size of the mRNAs for each protein were
identical, being approximately 1800 bases in length.
Phenobarbital induction of rat liver cytochromes P-450b and P-450e. Quantitation of specific RNAs by hybridization to synthetic oligodeoxyribonucleotide probes
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