Ferritin and superoxide-dependent lipid peroxidation

HOME

HELP

FEEDBACK

SUBSCRIPTIONS

Ferritin and superoxide-dependent lipid peroxidation

CE Thomas, LA Morehouse and SD Aust

Ferritin was found to promote the peroxidation of phospholipid liposomes, as evidenced by malondialdehyde formation, when incubated with xanthine oxidase, xanthine, and ADP. Activity was inhibited by superoxide dismutase but markedly stimulated by the addition of catalase. Xanthine oxidase-dependent iron release from ferritin, measured spectrophotometrically using the ferrous iron chelator 2,2'- dipyridyl, was also inhibited by superoxide dismutase, suggesting that superoxide can mediate the reductive release of iron from ferritin. Potassium superoxide in crown ether also promoted superoxide dismutase- inhibitable release of iron from ferritin. Catalase had little effect on the rate of iron release from ferritin; thus hydrogen peroxide appears to inhibit lipid peroxidation by preventing the formation of an initiating species rather than by inhibiting iron release from ferritin. EPR spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide was used to observe free radical production in this system. Addition of ferritin to the xanthine oxidase system resulted in loss of the superoxide spin trap adduct suggesting an interaction between superoxide and ferritin. The resultant spectrum was that of a hydroxyl radical spin trap adduct which was abolished by the addition of catalase. These data suggest that ferritin may function in vivo as a source of iron for promotion of superoxide-dependent lipid peroxidation. Stimulation of lipid peroxidation but inhibition of hydroxyl radical formation by catalase suggests that, in this system, initiation is not via an iron-catalyzed Haber-Weiss reaction.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

J. M. Fernandez-Real, J. M. Moreno, B. Chico, A. Lopez-Bermejo, and W. Ricart
Circulating Visfatin Is Associated With Parameters of Iron Metabolism in Subjects With Altered Glucose Tolerance
Diabetes Care, March 1, 2007; 30(3): 616 - 621.
[Abstract] [Full Text] [PDF]

G. Andonegui, S. M. Kerfoot, K. McNagny, K. V. J. Ebbert, K. D. Patel, and P. Kubes
Platelets express functional Toll-like receptor-4
Blood, October 1, 2005; 106(7): 2417 - 2423.
[Abstract] [Full Text] [PDF]

D. L. van der, D. E. Grobbee, M. Roest, J. J.M. Marx, H. A. Voorbij, and Y. T. van der Schouw
Serum Ferritin Is a Risk Factor for Stroke in Postmenopausal Women
Stroke, August 1, 2005; 36(8): 1637 - 1641.
[Abstract] [Full Text] [PDF]

T. Shang, S. Kotamraju, S. V. Kalivendi, C. J. Hillard, and B. Kalyanaraman
1-Methyl-4-phenylpyridinium-induced Apoptosis in Cerebellar Granule Neurons Is Mediated by Transferrin Receptor Iron-dependent Depletion of Tetrahydrobiopterin and Neuronal Nitric-oxide Synthase-derived Superoxide
J. Biol. Chem., April 30, 2004; 279(18): 19099 - 19112.
[Abstract] [Full Text] [PDF]

S. Xiong, H. She, H. Takeuchi, B. Han, J. F. Engelhardt, C. H. Barton, E. Zandi, C. Giulivi, and H. Tsukamoto
Signaling Role of Intracellular Iron in NF-kappa B Activation
J. Biol. Chem., May 9, 2003; 278(20): 17646 - 17654.
[Abstract] [Full Text] [PDF]

K. SATO, M. B. KADIISKA, A. J. GHIO, J. CORBETT, Y. C. FANN, S. M. HOLLAND, R. G. THURMAN, and R. P. MASON
In vivo lipid-derived free radical formation by NADPH oxidase in acute lung injury induced by lipopolysaccharide: a model for ARDS
FASEB J, November 1, 2002; 16(13): 1713 - 1720.
[Abstract] [Full Text] [PDF]

G. Andonegui, S. M. Goyert, and P. Kubes
Lipopolysaccharide-Induced Leukocyte-Endothelial Cell Interactions: A Role for CD14 Versus Toll-Like Receptor 4 Within Microvessels
J. Immunol., August 15, 2002; 169(4): 2111 - 2119.
[Abstract] [Full Text] [PDF]

S. Kotamraju, C. R. Chitambar, S. V. Kalivendi, J. Joseph, and B. Kalyanaraman
Transferrin Receptor-dependent Iron Uptake Is Responsible for Doxorubicin-mediated Apoptosis in Endothelial Cells. ROLE OF OXIDANT-INDUCED IRON SIGNALING IN APOPTOSIS
J. Biol. Chem., May 3, 2002; 277(19): 17179 - 17187.
[Abstract] [Full Text] [PDF]

R. J. Bergeron, W. R. Weimar, and J. Wiegand
Pharmacokinetics of Orally Administered Desferrithiocin Analogs in Cebus apella Primates
Drug Metab. Dispos., December 1, 1999; 27(12): 1496 - 1498.
[Abstract] [Full Text]

A. BESARAB, S. FRINAK, and J. YEE
An Indistinct Balance: The Safety and Efficacy of Parenteral IronTherapy
J. Am. Soc. Nephrol., September 1, 1999; 10(9): 2029 - 2043.
[Full Text]

W. J. Bartfay, F. Dawood, W. H. Wen, D. C. Lehotay, D. Hou, E. Bartfay, X. Luo, P. H. Backx, and P. P. Liu
Cardiac function and cytotoxic aldehyde production in a murine model of chronic iron-overload
Cardiovasc Res, September 1, 1999; 43(4): 892 - 900.
[Abstract] [Full Text] [PDF]

X. Xing, J. Baffic, and C. P. Sparrow
LDL oxidation by activated monocytes: characterization of the oxidized LDL and requirement for transition metal ions
J. Lipid Res., November 1, 1998; 39(11): 2201 - 2208.
[Abstract] [Full Text]

S. Kiechl, J. Willeit, G. Egger, W. Poewe, F. Oberhollenzer, and f. t. B. S. Group
Body Iron Stores and the Risk of Carotid Atherosclerosis : Prospective Results From the Bruneck Study
Circulation, November 18, 1997; 96(10): 3300 - 3307.
[Abstract] [Full Text]

C. K. Mukhopadhyay, E. Ehrenwald, and P. L. Fox
Ceruloplasmin Enhances Smooth Muscle Cell- and Endothelial Cell-mediated Low Density Lipoprotein Oxidation by a Superoxide-dependent Mechanism
J. Biol. Chem., June 21, 1996; 271(25): 14773 - 14778.
[Abstract] [Full Text] [PDF]

R. E. Kirschner and G. A. Fantini
Role of Iron in Reoxygenation Injury of Postischemic Skeletal Muscle
Perspectives in Vascular Surgery and Endovascular Therapy, January 1, 1992; 5(2): 69 - 83.
[PDF]