|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 260, Issue 6, 3477-3483, 03, 1985
VA Florio and PC Sternweis
The association of agonists with muscarinic receptors in membranes from
bovine brain was affected only slightly by guanine nucleotides. However,
solubilization of these membranes with deoxycholate and subsequent removal
of detergent resulted in a preparation of receptors with increased affinity
for agonists and a large increase in response to guanine nucleotides.
Chromatography of deoxycholate extracts of membranes on DEAE-Sephacel
resulted in the separation of receptors from 95% of the guanine
nucleotide-binding activity. Guanine nucleotides had no effect on the
binding of agonists to these resolved receptors. The effect of guanine
nucleotides was restored after the addition of either of two purified
guanine nucleotide-binding proteins from bovine brain. One of these
proteins, presumably brain GI, is composed of subunits with the same
molecular weights (alpha, 41,000; beta, 35,000; gamma, 11,000) and
functions as the inhibitory guanine nucleotide-binding protein isolated
from liver. The other protein, termed Go, is a novel guanine
nucleotide-binding protein that possesses a similar subunit composition
(alpha, 39,000; beta, 35,000; gamma, 11,000) but whose function is not yet
known. Addition of either protein to the resolved receptor preparation
increased agonist affinity by at least 10-20-fold, and low concentrations
of guanine nucleotides specifically reversed this effect. Reconstitution of
receptors with the resolved subunits of Go demonstrates that the beta
subunit alone had no effect on agonist binding, but that this subunit does
appear to enhance the effects observed with the alpha subunit alone.
Reconstitution of resolved muscarinic cholinergic receptors with purified GTP-binding proteins
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. R. Kerchner, R. L. Clay, G. McCleery, N. Watson, W. E. McIntire, C.-S. Myung, and J. C. Garrison Differential Sensitivity of Phosphatidylinositol 3-Kinase p110{gamma} to Isoforms of G Protein {beta}{gamma} Dimers J. Biol. Chem., October 22, 2004; 279(43): 44554 - 44562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Waelbroeck Activation of Guanosine 5'-[{gamma}-35S]thio-triphosphate Binding through M1 Muscarinic Receptors in Transfected Chinese Hamster Ovary Cell Membranes: 1. Mathematical Analysis of Catalytic G Protein Activation Mol. Pharmacol., April 1, 2001; 59(4): 875 - 885. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Jiang, K. Spicher, G. Boulay, Y. Wang, and L. Birnbaumer Most central nervous system D2 dopamine receptors are coupled to their effectors by Go PNAS, March 1, 2001; (2001) 51632598. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ohtaki, K. Ogi, Y. Masuda, K. Mitsuoka, Y. Fujiyoshi, C. Kitada, H. Sawada, H. Onda, and M. Fujino Expression, Purification, and Reconstitution of Receptor for Pituitary Adenylate Cyclase-activating Polypeptide. LARGE-SCALE PURIFICATION OF A FUNCTIONALLY ACTIVE G PROTEIN-COUPLED RECEPTOR PRODUCED IN SF9 INSECT CELLS J. Biol. Chem., June 19, 1998; 273(25): 15464 - 15473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jiang, M. S. Gold, G. Boulay, K. Spicher, M. Peyton, P. Brabet, Y. Srinivasan, U. Rudolph, G. Ellison, and L. Birnbaumer Multiple neurological abnormalities in mice deficient in the G protein Go PNAS, March 17, 1998; 95(6): 3269 - 3274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Vasudevan, W Han, Y Tan, Y Nie, D Li, K Shome, S. Watkins, E. Levitan, and G Romero The distribution and translocation of the G protein ADP-ribosylation factor 1 in live cells is determined by its GTPase activity J. Cell Sci., January 5, 1998; 111(9): 1277 - 1285. [Abstract] [PDF]
|
|
|
|
|
|
H. Yasuda, MargaretA. Lindorfer, KarenA. Woodfork, JuliaE. Fletcher, and JamesC. Garrison Role of the Prenyl Group on the G Protein gamma Subunit in Coupling Trimeric G Proteins to A1 Adenosine Receptors J. Biol. Chem., August 2, 1996; 271(31): 18588 - 18595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Butkerait, Y. Zheng, H. Hallak, T. E. Graham, H. A. Miller, K. D. Burris, P. B. Molinoff, and D. R. Manning Expression of the Human 5-Hydroxytryptamine[IMAGE] Receptor in Sf9 Cells J. Biol. Chem., August 4, 1995; 270(31): 18691 - 18699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. C. Ong, H. K. Yamane, K. B. Phan, H. K. W. Fong, D. Bok, R. H. Lee, and B. K.-K. Fung Molecular Cloning and Characterization of the G Protein [IMAGE] Subunit of Cone Photoreceptors J. Biol. Chem., April 14, 1995; 270(15): 8495 - 8500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-J. Tang, J.A. Iniguez-Lluhi, S. Mumby, and A.G. Gilman Regulation of Mammalian Adenylyl Cyclases by G-Protein {alpha} and {beta}{gamma} Subunits Cold Spring Harb Symp Quant Biol, January 1, 1992; 57(0): 135 - 144. [Abstract] [PDF]
|
|
|
|
 |
|
 N Gautam, M Baetscher, R Aebersold, and M. Simon A G protein gamma subunit shares homology with ras proteins Science, May 26, 1989; 244(4907): 971 - 974. [Abstract] [PDF]
|
|
|
|
|
|
A. Otero, G. Breitwieser, and G Szabo Activation of muscarinic potassium currents by ATP gamma S in atrial cells Science, October 21, 1988; 242(4877): 443 - 445. [Abstract] [PDF]
|
|
|
|
|
|
A Yatani, H Hamm, J Codina, M. Mazzoni, L Birnbaumer, and A. Brown A monoclonal antibody to the alpha subunit of Gk blocks muscarinic activation of atrial K+ channels Science, August 12, 1988; 241(4867): 828 - 831. [Abstract] [PDF]
|
|
|
|
|
|
M. Jiang, K. Spicher, G. Boulay, Y. Wang, and L. Birnbaumer Most central nervous system D2 dopamine receptors are coupled to their effectors by Go PNAS, March 13, 2001; 98(6): 3577 - 3582. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |