HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pandol, S. J. Articles by Schoeffield, M. S. Search for Related Content PubMed PubMed Citation Articles by Pandol, S. J. Articles by Schoeffield, M. S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 10, 4438-4444, 04, 1986

1,2-Diacylglycerol, protein kinase C, and pancreatic enzyme secretion

SJ Pandol and MS Schoeffield

To determine the role of 1,2-diacylglycerol (1,2-DAG) and protein kinase C in pancreatic enzyme secretion, we measured the effect of various pancreatic secretagogues on the cellular mass of 1,2-DAG and amylase release in dispersed pancreatic acini from the guinea pig. In addition, we measured the effect of a recently described protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) (Hidaka, H., Inagaki, M., Kawamoto, S., and Sasaki, Y. (1984) Biochemistry 23, 5036-5041), on secretagogue-stimulated amylase release from the acini. Cholecystokinin-octapeptide (CCK-OP), cholecystokinintetrapeptide, and carbachol each increased 1,2-DAG 2-3- fold but the increases occurred only with concentrations of these secretagogues that were supramaximal for amylase release and that had an inhibitory effect on stimulated amylase release. Supramaximal concentrations of bombesin stimulated only a small increase in 1,2-DAG and did not cause inhibition of stimulated amylase release. When the action of carbachol was terminated with atropine or CCK-OP with dibutyryl cyclic GMP, stimulated amylase release ceased immediately but cellular 1,2-DAG required at least 15 min to return to the basal level. Increasing cytosolic free Ca2+ with the Ca2+ ionophore, A23187, in Ca2+- containing incubation media augmented amylase release stimulated by 4 beta-phorbol 12-myristate 13-acetate but inhibited amylase release stimulated by CCK-OP, carbachol, and bombesin without decreasing the cellular content of 1,2-DAG. H-7 inhibited protein kinase C activity in a pancreatic homogenate but augmented amylase release from acini stimulated by either CCK-OP, carbachol, or 4 beta-phorbol 12-myristate 13-acetate. These findings indicate that 1,2-DAG and protein kinase C do not have a stimulatory role in pancreatic stimulus-secretion coupling but may have an inhibitory one.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Satoh, A. S. Gukovskaya, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol Ethanol sensitizes NF-{kappa}B activation in pancreatic acinar cells through effects on protein kinase C-{epsilon} Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G432 - G438. [Abstract] [Full Text] [PDF]

				A. Satoh, A. S. Gukovskaya, J. M. Nieto, J. H. Cheng, I. Gukovsky, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol PKC-{delta} and -{epsilon} regulate NF-{kappa}B activation induced by cholecystokinin and TNF-{alpha} in pancreatic acinar cells Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G582 - G591. [Abstract] [Full Text] [PDF]

				A. S. Gukovskaya, I. Gukovsky, Y. Jung, M. Mouria, and S. J. Pandol Cholecystokinin Induces Caspase Activation and Mitochondrial Dysfunction in Pancreatic Acinar Cells. ROLES IN CELL INJURY PROCESSES OF PANCREATITIS J. Biol. Chem., June 14, 2002; 277(25): 22595 - 22604. [Abstract] [Full Text] [PDF]

				Y. Hirohata, M. Fujii, Y. Okabayashi, Y. Nagashio, M. Tashiro, I. Imoto, T. Akiyama, and M. Otsuki Stimulatory effects of bilirubin on amylase release from isolated rat pancreatic acini Am J Physiol Gastrointest Liver Physiol, February 1, 2002; 282(2): G249 - G256. [Abstract] [Full Text] [PDF]

				B. Han and C. D. Logsdon CCK stimulates mob-1 expression and NF-kappa B activation via protein kinase C and intracellular Ca2+ Am J Physiol Cell Physiol, February 1, 2000; 278(2): C344 - C351. [Abstract] [Full Text] [PDF]

				H. Nishino, Y. Tsunoda, and C. Owyang Mammalian bombesin receptors are coupled to multiple signal transduction pathways in pancreatic acini Am J Physiol Gastrointest Liver Physiol, March 1, 1998; 274(3): G525 - G534. [Abstract] [Full Text] [PDF]