| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 261, Issue 17, 7581-7584, 06, 1986
R Friesel, WH Burgess, T Mehlman and T Maciag
Cellular receptors for endothelial cell growth factor (ECGF) have been
demonstrated on several cell types by binding of 125I-ECGF in a specific
and saturable manner (Schreiber, A. B., Kennedy, J., Kowalski, J., Friesel,
R., Mehlman, T., and Maciag, T. (1985) Proc. Natl. Acad. Sci. U. S. A. 82,
6138-6142). Here we report the covalent cross-linking of 125I-ECGF to a
polypeptide present on the surface of the plasma membrane of murine lung
capillary endothelial cells by the homobifunctional reagent, disuccinimidyl
suberate. Cross-linking of cell surface associated 125I-ECGF yields a major
polypeptide with an apparent molecular weight of 150,000. Experiments
demonstrated that the cross-linked polypeptide complex represents 125I-ECGF
covalently bound specifically to a cell surface receptor because: covalent
modification of the polypeptide was inhibited by excess, unlabeled ECGF;
preincubation of cells with unlabeled ECGF at 37 degrees C significantly
reduced cross-linking while incubation at 4 degrees C did not; other
polypeptide growth factors do not compete with 125I-ECGF for cross-linking
to the ECGF receptor; labeling of the polypeptide did not take place in the
absence of DSS; and cells previously shown to have a paucity of ECGF
receptors did not yield a cross-linked labeled receptor. These data suggest
that the mitogenic events mediated by ECGF occur after occupancy of the
specific cell surface polypeptide and suggest that these events are
relevant to ECGF-induced signal transduction across the endothelial cell
plasma membrane.
The characterization of the receptor for endothelial cell growth factor by covalent ligand attachment
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R Tabibiazar and S.G Rockson Angiogenesis and the ischaemic heart Eur. Heart J., June 1, 2001; 22(11): 903 - 918. [PDF]
|
|
|
|
 |
|
 P. Pecher and B. A. Schumacher Angiogenesis in ischemic human myocardium: clinical results after 3 years Ann. Thorac. Surg., May 1, 2000; 69(5): 1414 - 1419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schumacher, P. Pecher, B. U. von Specht, and Th. Stegmann Induction of Neoangiogenesis in Ischemic Myocardium by Human Growth Factors : First Clinical Results of a New Treatment of Coronary Heart Disease Circulation, February 24, 1998; 97(7): 645 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Neilson and R. Friesel Ligand-independent Activation of Fibroblast Growth Factor Receptors by Point Mutations in the Extracellular, Transmembrane, and Kinase Domains J. Biol. Chem., October 4, 1996; 271(40): 25049 - 25057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tanahashi, M. Suzuki, T. Imamura, and Y. Mitsui Identification of a 79-kDa Heparin-binding Fibroblast Growth Factor (FGF) Receptor in Rat Hepatocytes and Its Correlation with the Different Growth Responses to FGF-1 between Hepatocyte Subpopulations J. Biol. Chem., April 5, 1996; 271(14): 8221 - 8227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Neilson and R. E. Friesel Constitutive Activation of Fibroblast Growth Factor Receptor-2 by a Point Mutation Associated with Crouzon Syndrome J. Biol. Chem., November 3, 1995; 270(44): 26037 - 26040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fasol, B. Schumacher, K. Schlaudraff, K.-H. Hauenstein, and R. Seitelberger Experimental use of a modified fibrin glue to induce site-directed angiogenesis from the aorta to the heart J. Thorac. Cardiovasc. Surg., June 1, 1994; 107(6): 1432 - 1439. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Thompson, K. Anderson, J. DiPietro, J. Zwiebel, M Zametta, W. Anderson, and T Maciag Site-directed neovessel formation in vivo Science, September 9, 1988; 241(4871): 1349 - 1352. [Abstract] [PDF]
|
|
|
|
|
|
J Folkman and M Klagsbrun Angiogenic factors Science, January 23, 1987; 235(4787): 442 - 447. [Abstract] [PDF]
|
|
|
|
|
|
M Jaye, R Howk, W Burgess, G. Ricca, I. Chiu, M. Ravera, S. O'Brien, W. Modi, T Maciag, and W. Drohan Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization Science, August 1, 1986; 233(4763): 541 - 545. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
