HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mol, J. A. Articles by Hennemann, G. Search for Related Content PubMed PubMed Citation Articles by Mol, J. A. Articles by Hennemann, G. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 17, 7640-7643, Jun, 1986

Inhibition of iodothyronine transport into rat liver cells by a monoclonal antibody

JA Mol, EP Krenning, R Docter, J Rozing and G Hennemann

The role of the rat liver plasma membrane in the regulation of uptake and subsequent deiodination of thyroxine (T4) or the biologically active thyroid hormone 3,3',5-triiodothyronine (T3) was investigated. Here we report on the production of monoclonal antibodies raised against rat hepatocytes. Two antibodies were selected. Antibody ER-22 did bind to a Mr 52,000 membrane protein and inhibited the 1- and 5-min uptake of both T4 and T3 by primary cultured rat hepatocytes in a dose- dependent fashion. As the uptake of T4 and T3 depends on the presence of a sodium gradient over the plasma membrane, the inhibitory potency of ER-22 on the Na+,K+-ATPase activity was investigated. No inhibition of the uptake of 86Rb+ could be determined, indicating that antibody ER- 22 is not directed against the Na+,K+-ATPase but probably the carrier protein itself. Clearance of T3 from the medium and concomitant iodide production by cultured rat hepatocytes during a 20-h incubation in the presence of ER-22 were both inhibited by 50% with respect to a control incubation in the absence of monoclonal antibody, pointing to the importance of carrier-mediated transport in cellular uptake and metabolism of T3. A second monoclonal antibody did bind to two other plasma membrane proteins but did not inhibit transport of thyroid hormone.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Hennemann, R. Docter, E. C. H. Friesema, M. de Jong, E. P. Krenning, and T. J. Visser Plasma Membrane Transport of Thyroid Hormones and Its Role in Thyroid Hormone Metabolism and Bioavailability Endocr. Rev., August 1, 2001; 22(4): 451 - 476. [Abstract] [Full Text] [PDF]

				R. R. Cavalieri, L. A. Simeoni, S. W. Park, J. D. Baxter, B. F. Scharschmidt, R. C. J. Ribeiro, and N. Lomri Thyroid Hormone Export in Rat FRTL-5 Thyroid Cells and Mouse NIH-3T3 Cells Is Carrier-Mediated, Verapamil-Sensitive, and Stereospecific Endocrinology, November 1, 1999; 140(11): 4948 - 4954. [Abstract] [Full Text]

				R. Docter, E. C. H. Friesema, P. G. J. van Stralen, E. P. Krenning, M. E. Everts, T. J. Visser, and G. Hennemann Expression of Rat Liver Cell Membrane Transporters for Thyroid Hormone in Xenopus laevis Oocytes Endocrinology, May 1, 1997; 138(5): 1841 - 1846. [Abstract] [Full Text] [PDF]