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J. Biol. Chem., Vol. 261, Issue 17, 7771-7777, 06, 1986
FH Chilton and RC Murphy
Initial incorporation and subsequent remodeling of 16 phosphoglyceride
molecular species containing arachidonate in the human neutrophil have been
studied. Neutrophils were pulse-labeled with [3H]arachidonic acid (AA) for
5 min, then phospholipids were analyzed either at this time point or after
a subsequent 120-min incubation. [3H]AA was found to be incorporated into
phosphoglycerides phosphatidylinositol (PI) greater than
phosphatidylcholine (PC) greater than phosphatidylethanolamine (PE) by 5
min. Incorporation of [3H]AA was not related to pool size, but reflected an
increase in phosphoglyceride turnover. Following the 120-min incubation,
only PE gained a significant amount of labeled arachidonate. Specific
activity analysis revealed that PI contained the highest labeled/unlabeled
ratio at both 5 min and 120 min. After the initial 5-min pulse, the
majority of [3H]arachidonate was incorporated into
1-acyl-2-[3H]arachidonoyl-sn-glycero-3-PC, -PE, and -PI showing no
preference for fatty acyl chains at the sn-1 position. However, [3H]AA was
remodeled into 1-alkyl-acyl-and 1-alk-1-enyl-acyl-sn-glycero-3-PC and -PE
molecular species in those neutrophils incubated for the additional 120
min. Specific activities of [3H]AA within all diacyl molecular species were
initially higher relative to those alkyl-acyl and alk-1-enyl-acyl molecular
species, but for PC and PE became more uniform as label shifted into ether
and plasmalogen pools during the additional 120-min incubation. In
contrast, the specific activity of 1-
stearoyl-2-arachidonoyl-sn-glycero-3-PI remained constant throughout the
120-min incubation.
Remodeling of arachidonate-containing phosphoglycerides within the human neutrophil
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