HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gray, J. Articles by Carroll, E. J. Search for Related Content PubMed PubMed Citation Articles by Gray, J. Articles by Carroll, E. J., Jr Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 20, 9282-9288, Jul, 1986

Resolution and characterization of a major protein of the sea urchin hyaline layer

J Gray, R Justice, GM Nagel and EJ Carroll Jr

A major protein component of the gel-like, embryonic hyaline layer of Strongylocentrotus purpuratus has been purified and characterized. The protein retains the ability to form an insoluble gel in the presence of specific divalent cations, a property characteristic of the hyaline material. Using a light scattering assay developed to measure the initial rate of hyalin gelation, we have been able to show that calcium alone is capable of initiating this reaction but that calcium and magnesium are synergistic in their effect. In the absence of divalent cations, the major hyalin protein has a molecular weight of 9.2 +/- 0.5 X 10(5) and a sedimentation coefficient of 11.6 S; these and other data indicate that the protein assumes a very elongated, rod-like structure in solution. Smaller amounts of two additional proteins, 8.8 and 6.5 S, are present in the hyalin fraction when the jelly coat and vitelline layer are subjected to a more stringent acid treatment early in the isolation procedure.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Hoodbhoy, E. J. Carroll Jr., and P. Talbot Relationship Between p62 and p56, Two Proteins of the Mammalian Cortical Granule Envelope, and Hyalin, the Major Component of the Echinoderm Hyaline Layer, in Hamsters Biol Reprod, April 1, 2000; 62(4): 979 - 987. [Abstract] [Full Text]