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J. Biol. Chem., Vol. 261, Issue 6, 2496-2498, Feb, 1986

Availability of hyperacetylated H4 histone in intact nucleosomes to specific antibodies

U Pfeffer, N Ferrari and G Vidali

Specific antibodies against the tetra-acetylated form of H4 histone have been elicited in the rabbit. They do not cross-react with the non- , mono-, and di-acetylated forms of the histone molecule but a slight cross-reactivity with the tri-acetylated form of H4 histone is observed. Our studies also show that hyperacetylated H4 histones are recognized by the antibodies in intact nucleosomes.
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