HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuroda, M. Articles by Simpson, I. A. Search for Related Content PubMed PubMed Citation Articles by Kuroda, M. Articles by Simpson, I. A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 1, 245-253, 01, 1987

Regulation of insulin-stimulated glucose transport in the isolated rat adipocyte. cAMP-independent effects of lipolytic and antilipolytic agents

M Kuroda, RC Honnor, SW Cushman, C Londos and IA Simpson

This paper examines the modulation of insulin-stimulated glucose transport activity in rat adipose cells by ligands for receptors (R) that mediate stimulation (Rs; lipolytic) or inhibition (Ri; antilipolytic) of adenylate cyclase. The changes in glucose transport activity and cAMP, as assessed by 3-O-methylglucose uptake and (-/+) cAMP-dependent protein kinase (A-kinase) activity ratios, respectively, were monitored under conditions that maintain steady-state A-kinase activity ratios (Honnor, R. C., Dhillon, G. S., and Londos, C. (1985) J. Biol. Chem. 260, 15122-15129). Removal of endogenous adenosine with adenosine deaminase decreased insulin-stimulated glucose transport activity by approximately 30%, which was prevented or restored with Ri agonists such as phenylisopropyladenosine, nicotinic acid, and prostaglandin E1. These changes in transport activity were not accompanied by changes in A-kinase activity ratios, indicating that Ri- mediated effects on transport are independent of cAMP changes. Addition of an Rs ligand, isoproterenol, in the presence of adenosine increased kinase activity but did not change glucose transport activity. Conversely, upon removal of adenosine, addition of Rs ligands such as isoproterenol, adrenocorticotropic hormone, or glucagon strongly inhibited transport (approximately 50%) and stimulated kinase activity. However, subsequent addition of phenylisopropyladenosine nearly restored transport activity without alteration of A-kinase activity. These data and additional kinetic experiments suggest that Rs-mediated glucose transport modulations are also independent of cAMP. The interchangeability of ligands for both Rs and Ri receptors in modulating transport activity suggests that these cAMP-independent effects are mediated by the stimulatory (Ns) and inhibitory (Ni) guanyl nucleotide-binding regulatory proteins of adenylate cyclase. All Rs-and Ri-induced changes in transport activity occurred without a change in glucose transporter distribution, as assessed by D-glucose-inhibitable cytochalasin B binding, suggesting that Rs and Ri ligands modulate the intrinsic activity of the glucose transporter present in the plasma membrane.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Viswanadha and C. Londos Optimized conditions for measuring lipolysis in murine primary adipocytes J. Lipid Res., August 1, 2006; 47(8): 1859 - 1864. [Abstract] [Full Text] [PDF]

				C. Gonzalez-Yanes, A. Serrano, F. J. Bermudez-Silva, M. Hernandez-Dominguez, M. A. Paez-Ochoa, F. R. de Fonseca, and V. Sanchez-Margalet Oleylethanolamide impairs glucose tolerance and inhibits insulin-stimulated glucose uptake in rat adipocytes through p38 and JNK MAPK pathways Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E923 - E929. [Abstract] [Full Text] [PDF]

				A. H. Mulder, C. J. Tack, A. J. Olthaar, P. Smits, F. C. G. J. Sweep, and R. R. Bosch Adrenergic receptor stimulation attenuates insulin-stimulated glucose uptake in 3T3-L1 adipocytes by inhibiting GLUT4 translocation Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E627 - E633. [Abstract] [Full Text] [PDF]

				C. Schoelch, J. Kuhlmann, M. Gossel, G. Mueller, C. Neumann-Haefelin, U. Belz, J. Kalisch, G. Biemer-Daub, W. Kramer, H.-P. Juretschke, et al. Characterization of Adenosine-A1 Receptor-Mediated Antilipolysis in Rats by Tissue Microdialysis, 1H-Spectroscopy, and Glucose Clamp Studies Diabetes, July 1, 2004; 53(7): 1920 - 1926. [Abstract] [Full Text] [PDF]

				R. T. Watson, M. Kanzaki, and J. E. Pessin Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes Endocr. Rev., April 1, 2004; 25(2): 177 - 204. [Abstract] [Full Text] [PDF]

				L. C. C. Navegantes, M. Sjostrand, S. Gudbjornsdottir, L. Strindberg, M. Elam, and P. Lonnroth Regulation and Counterregulation of Lipolysis in Vivo: Different Roles of Sympathetic Activation and Insulin J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5515 - 5520. [Abstract] [Full Text] [PDF]

				M. J. Watt and M. Hargreaves Effect of epinephrine on glucose disposal during exercise in humans: role of muscle glycogen Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E578 - E583. [Abstract] [Full Text] [PDF]

				F. S. L. Thong and T. E. Graham Caffeine-induced impairment of glucose tolerance is abolished by beta -adrenergic receptor blockade in humans J Appl Physiol, June 1, 2002; 92(6): 2347 - 2352. [Abstract] [Full Text] [PDF]

				G. B. Keijzers, B. E. De Galan, C. J. Tack, and P. Smits Caffeine Can Decrease Insulin Sensitivity in Humans Diabetes Care, February 1, 2002; 25(2): 364 - 369. [Abstract] [Full Text] [PDF]

				A. Bose, A. D. Cherniack, S. E. Langille, S. M. C. Nicoloro, J. M. Buxton, J. G. Park, A. Chawla, and M. P. Czech G{alpha}11 Signaling through ARF6 Regulates F-Actin Mobilization and GLUT4 Glucose Transporter Translocation to the Plasma Membrane Mol. Cell. Biol., August 1, 2001; 21(15): 5262 - 5275. [Abstract] [Full Text] [PDF]

				J. S. Bogan, A. E. McKee, and H. F. Lodish Insulin-Responsive Compartments Containing GLUT4 in 3T3-L1 and CHO Cells: Regulation by Amino Acid Concentrations Mol. Cell. Biol., July 15, 2001; 21(14): 4785 - 4806. [Abstract] [Full Text] [PDF]

				A. Lorenzen, C. Stannek, H. Lang, V. Andrianov, I. Kalvinsh, and U. Schwabe Characterization of a G Protein-Coupled Receptor for Nicotinic Acid Mol. Pharmacol., February 1, 2001; 59(2): 349 - 357. [Abstract] [Full Text]

				E. M. Cornford, E. V. Nguyen, and E. M. Landaw Acute upregulation of blood-brain barrier glucose transporter activity in seizures Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1346 - H1354. [Abstract] [Full Text] [PDF]

				S. F. Hausdorff, D. C. Fingar, K. Morioka, L. A. Garza, E. L. Whiteman, S. A. Summers, and M. J. Birnbaum Identification of Wortmannin-sensitive Targets in 3T3-L1 Adipocytes. DISSOCIATION OF INSULIN-STIMULATED GLUCOSE UPTAKE AND GLUT4 TRANSLOCATION J. Biol. Chem., August 27, 1999; 274(35): 24677 - 24684. [Abstract] [Full Text] [PDF]

				S. Takasuga, T. Katada, M. Ui, and O. Hazeki Enhancement by Adenosine of Insulin-induced Activation of Phosphoinositide 3-Kinase and Protein Kinase B in Rat Adipocytes J. Biol. Chem., July 9, 1999; 274(28): 19545 - 19550. [Abstract] [Full Text] [PDF]