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J. Biol. Chem., Vol. 262, Issue 1, 63-68, Jan, 1987
P Regnier, M Grunberg-Manago and C Portier
The pnp gene is located at 69 min on the Escherichia coli chromosome
adjacent to the rpsO gene which encodes the ribosomal protein S15. In this
paper, we present the sequence of a 3030-nucleotide DNA fragment containing
the open reading frames coding for ribosomal protein S15 and polynucleotide
phosphorylase. Translation of pnp is initiated by 5'-UUG- 3' codon
separated by 7 nucleotides from a good ribosome binding site. Codon usage
in this gene is typical of highly expressed proteins of E. coli. Some of
the transcripts of the pnp gene terminate just after the stem of the
terminator t2 visible in the nucleotide sequence. However, a very strong
read-through occurs at this site, thus permitting many of the pnp
transcripts to extend beyond this transcription terminator. We also
describe the primary structure homologies between a 69-amino-acid stretch
of polynucleotide phosphorylase and the four homologous stretches of
ribosomal protein S1 which form its RNA binding site. The possibility that
this 69-amino-acid stretch constitutes the polynucleotide binding domain of
polynucleotide phosphorylase is discussed.
Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1
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