| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 10, 4592-4596, 04, 1987
PS Low, DP Allen, TF Zioncheck, P Chari, BM Willardson, RL Geahlen and ML Harrison
The cytoplasmic domain of band 3 (cdb3) of the human erythrocyte membrane
is a good substrate of endogenous and exogenous protein- tyrosine kinases.
Because one site of tyrosine phosphorylation is within the glycolytic
enzyme/hemoglobin-binding region at the N terminus of the polypeptide, we
have investigated whether tyrosine phosphorylation of cdb3 might influence
its interaction with the above peripheral proteins. Using p40, a
protein-tyrosine kinase isolated from bovine thymus, we demonstrate that
aldolase binding to cdb3 linked to Affi-Gel 15 is significantly inhibited
by phosphorylation of the immobilized band 3. Importantly, upon
dephosphorylation of the gel with acid phosphatase, aldolase binding
returns to prephosphorylated values. Similarly, cdb3 phosphorylation was
found to inhibit glyceraldehyde-3- phosphate dehydrogenase,
phosphofructokinase, and hemoglobin binding to immobilized cdb3. In the
converse experiment, untreated soluble cdb3 was shown to bind to
immobilized aldolase, whereas phosphorylated cdb3 (approximately equal to
1.8 mol of Pi/mol of cdb3) did not. Furthermore, phosphorylated cdb3 was
unable to inhibit aldolase catalysis, whereas untreated cdb3, as shown
previously by others, was a potent inhibitor. Taken together, these results
demonstrate that phosphorylation of cdb3 on tyrosine residues inhibits
peripheral protein binding at the polypeptide's N terminus. In view of the
known effect of glycolytic enzyme binding to band 3 on catalytic activity,
tyrosine phosphorylation of band 3 may modulate glycolysis in vivo.
Tyrosine phosphorylation of band 3 inhibits peripheral protein binding
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Chu, A. Breite, P. Ciraolo, R. S. Franco, and P. S. Low Characterization of the deoxyhemoglobin binding site on human erythrocyte band 3: implications for O2 regulation of erythrocyte properties Blood, January 15, 2008; 111(2): 932 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. BRAGADIN, F. ION-POPA, G. CLARI, and L. BORDIN SHP-1 Tyrosine Phosphatase in Human Erythrocytes Ann. N.Y. Acad. Sci., January 1, 2007; 1095(1): 193 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perrotta, A. Borriello, A. Scaloni, L. De Franceschi, A. M. Brunati, F. Turrini, V. Nigro, E. Miraglia del Giudice, B. Nobili, M. L. Conte, et al. The N-terminal 11 amino acids of human erythrocyte band 3 are critical for aldolase binding and protein phosphorylation: implications for band 3 function Blood, December 15, 2005; 106(13): 4359 - 4366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Jennings Evidence for a Second Binding/Transport Site for Chloride in Erythrocyte Anion Transporter AE1 Modified at Glutamate 681 Biophys. J., April 1, 2005; 88(4): 2681 - 2691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W Flatman Activation of ferret erythrocyte Na+-K+-2Cl- cotransport by deoxygenation J. Physiol., March 1, 2005; 563(2): 421 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Campanella, H. Chu, and P. S. Low Assembly and regulation of a glycolytic enzyme complex on the human erythrocyte membrane PNAS, February 15, 2005; 102(7): 2402 - 2407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bordin, A. M. Brunati, A. Donella-Deana, B. Baggio, A. Toninello, and G. Clari Band 3 is an anchor protein and a target for SHP-2 tyrosine phosphatase in human erythrocytes Blood, June 17, 2002; 100(1): 276 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Bottini, F Gloria-Bottini, M La Torre, and N Lucarini The genetics of signal transduction and the effect of smoking on intrauterine growth Int. J. Epidemiol., April 1, 2001; 30(2): 400 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Brunati, L. Bordin, G. Clari, P. James, M. Quadroni, E. Baritono, L. A. Pinna, and A. Donella-Deana Sequential phosphorylation of protein band 3 by Syk and Lyn tyrosine kinases in intact human erythrocytes: identification of primary and secondary phosphorylation sites Blood, August 15, 2000; 96(4): 1550 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gibson, A. Cossins, and J. Ellory Oxygen-sensitive membrane transporters in vertebrate red cells J. Exp. Biol., January 5, 2000; 203(9): 1395 - 1407. [Abstract] [PDF]
|
|
|
|
 |
|
 P. G. Lloyd and C. D. Hardin Role of microtubules in the regulation of metabolism in isolated cerebral microvessels Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1250 - C1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Musch, E. M. Davis-Amaral, K. L. Leibowitz, and L. Goldstein Hypotonic-stimulated taurine efflux in skate erythrocytes: regulation by tyrosine phosphatase activity Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1677 - R1686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Keshvara, C. Isaacson, M. L. Harrison, and R. L. Geahlen Syk Activation and Dissociation from the B-cell Antigen Receptor Is Mediated by Phosphorylation of Tyrosine 130 J. Biol. Chem., April 18, 1997; 272(16): 10377 - 10381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Wang, M. Tao, T. Wei, and P. S. Low Identification of the Major Casein Kinase I Phosphorylation Sites on Erythrocyte Band 3 Blood, April 15, 1997; 89(8): 3019 - 3024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Latour, L. M.L. Chow, and A. Veillette Differential Intrinsic Enzymatic Activity of Syk and Zap-70 Protein-tyrosine Kinases J. Biol. Chem., September 13, 1996; 271(37): 22782 - 22790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Peters, M. T. Furlong, D. J. Asai, M. L. Harrison, and R. L. Geahlen Syk, Activated by Cross-linking the B-cell Antigen Receptor, Localizes to the Cytosol Where It Interacts with and Phosphorylates alpha-Tubulin on Tyrosine J. Biol. Chem., March 1, 1996; 271(9): 4755 - 4762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Wang, R. Moriyama, C. R. Lombardo, and P. S. Low Partial Characterization of the Cytoplasmic Domain of Human Kidney Band 3 J. Biol. Chem., July 28, 1995; 270(30): 17892 - 17897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhou and P. S. Low Characterization of the Reversible Conformational Equilibrium in the Cytoplasmic Domain of Human Erythrocyte Membrane Band 3 J. Biol. Chem., October 5, 2001; 276(41): 38147 - 38151. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
