HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McKearin, D. M. Articles by Shapiro, D. J. Search for Related Content PubMed PubMed Citation Articles by McKearin, D. M. Articles by Shapiro, D. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 11, 4939-4942, Apr, 1987

Estrogen induces transcription of the Xenopus laevis serum retinol- binding protein gene

DM McKearin, MC Barton, MJ Keller and DJ Shapiro

We have isolated and sequenced a full-length cDNA cloned tentatively identified as encoding Xenopus laevis serum retinol-binding protein (RBP) mRNA. The derived amino acid sequence of the Xenopus protein is 63% homologous to the sequence of the human RBP. 17 of 19 amino acids identified as critical in the retinol-binding pocket of human RBP are identical or conservative replacements in the Xenopus protein. The RBP cDNA clone has been used as a hybridization probe to demonstrate that administration of estradiol-17 beta to male X. laevis induces hepatic RBP mRNA 10-fold from its constitutive in vivo level of 1,800 molecules/cell to approximately 18,000 molecules/cell. Using a simplified method for determining relative rates of gene transcription, we demonstrate an estrogen-mediated increase in the rate of RBP gene transcription. These quantitative data provide the first demonstration that a steroid hormone regulates the levels of vertebrate retinol- binding protein mRNA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Bellovino, T. Morimoto, E. Mengheri, G. Perozzi, I. Garaguso, F. Nobili, and S. Gaetani Unique Biochemical Nature of Carp Retinol-binding Protein. N-LINKED GLYCOSYLATION AND UNCLEAVABLE NH2-TERMINAL SIGNAL PEPTIDE J. Biol. Chem., April 20, 2001; 276(17): 13949 - 13956. [Abstract] [Full Text] [PDF]