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J. Biol. Chem., Vol. 262, Issue 11, 5188-5196, 04, 1987
EF Nemeth and A Scarpa
The concentration of intracellular free Ca2+ ([Ca2+]i) was measured in
dissociated bovine parathyroid cells using the fluorescent indicator quin-2
or fura-2. Small increases in the concentration of extracellular Ca2+
produced relatively slow, monophasic increases in [Ca2+]i in quin- 2-loaded
cells, but rapid and transient increases followed by lower, yet sustained
(steady-state), [Ca2+]i increases in fura-2-loaded cells. The different
patterns of change in [Ca2+]i reported by quin-2 and fura- 2 appear to
result from the greater intracellular Ca2+-buffering capacity present
within quin-2-loaded cells, which tends to damp rapid and transient changes
in [Ca2+]i. In fura-2-loaded parathyroid cells, other divalent cations
(Mg2+, Sr2+, Ba2+) also evoked transient increases in [Ca2+]i, and their
competitive interactions suggest that they all affect Ca2+ transients by
acting on a common site. In contrast, divalent cations failed to cause
increases in steady-state levels of cytosolic Ca2+. Low concentrations of
La3+ (0.5-10 microM) depressed steady-state levels of cytosolic Ca2+
elicited by extracellular Ca2+ but were without effect on transient
increases in [Ca2+]i elicited by extracellular Ca2+, Mg2+ or Sr2+,
suggesting that increases in the steady-state [Ca2+]i arise from the influx
of extracellular Ca2+. Mg2+- and Sr2+-induced cytosolic Ca2+ transients
persisted in the absence of extracellular Ca2+ but were abolished by
pretreatment with ionomycin. These results show that cytosolic Ca2+
transients arise from the mobilization of cellular Ca2+ from a
nonmitochondrial pool. Extracellular divalent cations thus appear to act at
some site on the surface of the cell, and this site can be considered a
"Ca2+ receptor" which enables the parathyroid cell to detect small changes
in the concentration of extracellular Ca2+.
Rapid mobilization of cellular Ca2+ in bovine parathyroid cells evoked by extracellular divalent cations. Evidence for a cell surface calcium receptor
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