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J. Biol. Chem., Vol. 262, Issue 12, 5445-5447, Apr, 1987

One-electron reduction of the oxyform of 2,4-diacetyldeuterocytochrome P-450cam

K Kobayashi, M Amano, Y Kanbara and K Hayashi

The reaction of the hydrated electron with a ferrous oxygenated form of modified cytochrome P-450cam, containing 2,4-diacetyldeuteroheme, was investigated by the use of pulse radiolysis. The ferrous oxygenated form of this enzyme was reduced by hydrated electrons to form the product, which exhibits absorption maximum at 470 and 370 nm. From the spectrum obtained, the oxidation state of the product is discussed in relation to the higher oxidation states of chloroperoxidase.
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