| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 15, 6951-6954, May, 1987
RA Smith and C Baglioni
Natural human and recombinant human and murine tumor necrosis factors (TNF)
were fractionated by gel filtration chromatography on Sephadex G- 75. The
active form of TNF was identified by its inhibitory activity in receptor
binding assays with HeLa cells and was eluted as a protein of Mr
approximately 55,000. Radioiodinated human and murine TNF were fractionated
by gel filtration into a major peak of Mr approximately 55,000,
corresponding to a trimer, and a minor peak of Mr approximately 17,000,
corresponding to a monomer. Binding assays showed that the timer was at
least 8-fold more active than the monomer. The human TNF partially
dissociated into monomers upon addition of the nonionic detergent Triton
X-100. Isolated monomers showed low binding affinity (KD = 70 nM) and
reduced cytotoxicity, whereas trimers showed high binding affinity (KD = 90
pM) and cytotoxicity. When 125I-TNF was bound to cells, no release of
monomer was detectable, suggesting that the trimer could directly bind to
cellular receptors without dissociating into subunits. Further evidence for
such binding was obtained by cross- linking 125I-TNF trimers with bis[2-
(succinimidooxycarbonyloxy)ethyl]sulfone. These trimers were bound to HeLa
cells, could be dissociated from cellular receptors, and elicited a
cytotoxic response. These results show that trimers, whether native or
cross-linked, bind to receptors and are the biologically active form of
TNF.
The active form of tumor necrosis factor is a trimer
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. D. McKenna, G. Feger, C. Kelton, M. Yang, V. Ardissone, R. Cirillo, P.-A. Vitte, X. Jiang, and R. K. Campbell Tumor Necrosis Factor (TNF)-Soluble High-Affinity Receptor Complex as a TNF Antagonist J. Pharmacol. Exp. Ther., August 1, 2007; 322(2): 822 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Berry, B. Hargadon, M. Shelley, D. Parker, D. E. Shaw, R. H. Green, P. Bradding, C. E. Brightling, A. J. Wardlaw, and I. D. Pavord Evidence of a Role of Tumor Necrosis Factor {alpha} in Refractory Asthma N. Engl. J. Med., February 16, 2006; 354(7): 697 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rabu, A. Quemener, Y. Jacques, K. Echasserieau, P. Vusio, and F. Lang Production of Recombinant Human Trimeric CD137L (4-1BBL): CROSS-LINKING IS ESSENTIAL TO ITS T CELL CO-STIMULATION ACTIVITY J. Biol. Chem., December 16, 2005; 280(50): 41472 - 41481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Faber, M. Bette, M. A. R. Preuss, R. Pulmanausahakul, J. Rehnelt, M. J. Schnell, B. Dietzschold, and E. Weihe Overexpression of Tumor Necrosis Factor Alpha by a Recombinant Rabies Virus Attenuates Replication in Neurons and Prevents Lethal Infection in Mice J. Virol., December 15, 2005; 79(24): 15405 - 15416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sacchi, A. Gasparri, F. Curnis, M. Bellone, and A. Corti Crucial Role for Interferon {gamma} in the Synergism between Tumor Vasculature-Targeted Tumor Necrosis Factor {alpha} (NGR-TNF) and Doxorubicin Cancer Res., October 1, 2004; 64(19): 7150 - 7155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Nielsen, J. Sauer, J. Backlund, B. Voldborg, K. Gregorius, S. Mouritsen, and T. Bratt Insertion of Foreign T Cell Epitopes in Human Tumor Necrosis Factor {alpha} with Minimal Effect on Protein Structure and Biological Activity J. Biol. Chem., August 6, 2004; 279(32): 33593 - 33600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Curnis, A. Gasparri, A. Sacchi, R. Longhi, and A. Corti Coupling Tumor Necrosis Factor-{alpha} with {alpha}V Integrin Ligands Improves Its Antineoplastic Activity Cancer Res., January 15, 2004; 64(2): 565 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schwamberger, P. Hammerl, E. Ferber, M. Freudenberg, and C. Galanos TNF revisited: TNF-independent antitumor activity in sera of mice sensitized with Propionibacterium acnes and challenged with lipopolysaccharide J. Leukoc. Biol., December 1, 2003; 74(6): 1056 - 1063. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ikeda, M. Kasai, J. Suzuki, H. Kuroyama, S. Seki, M. Utsuyama, and K. Hirokawa Multimerization of the Receptor Activator of Nuclear Factor-{kappa}B Ligand (RANKL) Isoforms and Regulation of Osteoclastogenesis J. Biol. Chem., November 21, 2003; 278(47): 47217 - 47222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Fourie, F. Coles, V. Moreno, and L. Karlsson Catalytic Activity of ADAM8, ADAM15, and MDC-L (ADAM28) on Synthetic Peptide Substrates and in Ectodomain Cleavage of CD23 J. Biol. Chem., August 15, 2003; 278(33): 30469 - 30477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Coward, Y. Okayama, H. Sagara, S. J. Wilson, S. T. Holgate, and M. K. Church NF-{kappa}B and TNF-{alpha}: A Positive Autocrine Loop in Human Lung Mast Cells? J. Immunol., November 1, 2002; 169(9): 5287 - 5293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D W Y Ho, S T Fan, J To, Y H Woo, Z Zhang, C Lau, and J Wong Selective plasma filtration for treatment of fulminant hepatic failure induced by D-galactosamine in a pig model Gut, June 1, 2002; 50(6): 869 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Curnis, G. Arrigoni, A. Sacchi, L. Fischetti, W. Arap, R. Pasqualini, and A. Corti Differential Binding of Drugs Containing the NGR Motif to CD13 Isoforms in Tumor Vessels, Epithelia, and Myeloid Cells Cancer Res., February 1, 2002; 62(3): 867 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Comini, T. Bachetti, A. Corti, and R. Ferrari A reply Eur. Heart J., May 2, 2000; 21(10): 857 - 857. [PDF]
|
|
|
|
 |
|
 N. S. Postma, R. C. Hermsen, D. J. A. Crommelin, W. M. C. Eling, and J. Zuidema Thiolated Recombinant Human Tumor Necrosis Factor-Alpha Protects against Plasmodium berghei K173-Induced Experimental Cerebral Malaria in Mice Antimicrob. Agents Chemother., May 1, 1999; 43(5): 1027 - 1033. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Pan, A. J. Kastin, R. L. Bell, and R. D. Olson Upregulation of Tumor Necrosis Factor alpha Transport across the Blood-Brain Barrier after Acute Compressive Spinal Cord Injury J. Neurosci., May 1, 1999; 19(9): 3649 - 3655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Postma, D. J. A. Crommelin, W. M. C. Eling, and J. Zuidema Treatment with Liposome-Bound Recombinant Human Tumor Necrosis Factor-alpha Suppresses Parasitemia and Protects against Plasmodium berghei k173-Induced Experimental Cerebral Malaria in Mice J. Pharmacol. Exp. Ther., January 1, 1999; 288(1): 114 - 120. [Abstract] [Full Text]
|
|
|
|
|
|
G. J. F. Ding, P. A. Fischer, R. C. Boltz, J. A. Schmidt, J. J. Colaianne, A. Gough, R. A. Rubin, and D. K. Miller Characterization and Quantitation of NF-kappa B Nuclear Translocation Induced by Interleukin-1 and Tumor Necrosis Factor-alpha . DEVELOPMENT AND USE OF A HIGH CAPACITY FLUORESCENCE CYTOMETRIC SYSTEM J. Biol. Chem., October 30, 1998; 273(44): 28897 - 28905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Sack, U. Burkhardt, M. Borte, H. Schädlich, K. Berg, and F. Emmrich Age-Dependent Levels of Select Immunological Mediators in Sera of Healthy Children Clin. Vaccine Immunol., January 1, 1998; 5(1): 28 - 32. [Abstract] [Full Text]
|
|
|
|
|
|
A. Al-Shamkhani, S. Mallett, M. H. Brown, W. James, and A. N. Barclay Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells J. Biol. Chem., February 21, 1997; 272(8): 5275 - 5282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Adam, D. Adam, and M. Krönke Cross-linking of the p55 Tumor Necrosis Factor Receptor Cytoplasmic Domain by a Dimeric Ligand Induces Nuclear Factor-[IMAGE]B and Mediates Cell Death J. Biol. Chem., July 21, 1995; 270(29): 17482 - 17487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Mazzei, M. D. Edgerton, C. Losberger, S. Lecoanet-Henchoz, P. Graber, A. Durandy, J.-F. Gauchat, A. Bernard, B. Allet, and J.-Y. Bonnefoy Recombinant Soluble Trimeric CD40 Ligand Is Biologically Active J. Biol. Chem., March 31, 1995; 270(13): 7025 - 7028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C.-H. Chen, G. C. DuBois, and M.-J. Chen Mapping the Domain(s) Critical for the Binding of Human Tumor Necrosis Factor-alpha to Its Two Receptors J. Biol. Chem., February 10, 1995; 270(6): 2874 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kagan, R. Baldwin, D Munoz, and B. Wisnieski Formation of ion-permeable channels by tumor necrosis factor-alpha Science, March 13, 1992; 255(5050): 1427 - 1430. [Abstract] [PDF]
|
|
|
|
|
|
C. Smith, T Davis, D Anderson, L Solam, M. Beckmann, R Jerzy, S. Dower, D Cosman, and R. Goodwin A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins Science, May 25, 1990; 248(4958): 1019 - 1023. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
