J. Biol. Chem., Vol. 262, Issue 15, 7001-7005, May, 1987

Structural analysis of the locus containing the human C-reactive protein gene and its related pseudogene

ND Goldman, T Liu and KJ Lei

The gene for human C-reactive protein (CRP) is mapped within a 34- kilobase pair genomic DNA segment identified by chromosome walking through overlapping DNA fragments cloned into a lambda phage library. Within 16 kilobase pairs upstream and downstream of the locus for the authentic CRP gene, only one other sequence homologous to that for CRP could be found. Sequencing analysis indicates this sequence to be a pseudogene with 50-80% region-specific homology. Comparison of the authentic CRP gene cloned from genomic DNA libraries independently prepared from three patients indicates no difference in the 5' and 3' flanking region, promoter region, or coding sequence. Only a polymorphism in the length of the poly(GT) stretch located in the intron is observed. There appears to be only one gene locus and copy per haploid chromosome for the authentic CRP gene and its pseudogene.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. G. Hage and A. J. Szalai C-Reactive Protein Gene Polymorphisms, C-Reactive Protein Blood Levels, and Cardiovascular Disease Risk J. Am. Coll. Cardiol., September 18, 2007; 50(12): 1115 - 1122. [Abstract] [Full Text] [PDF]

				C. Kluft and M. P.M. de Maat Genetics of C-Reactive Protein: New Possibilities and Complications Arterioscler. Thromb. Vasc. Biol., November 1, 2003; 23(11): 1956 - 1959. [Full Text] [PDF]