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J. Biol. Chem., Vol. 262, Issue 17, 8128-8130, Jun, 1987
Y Endo and K Tsurugi
The modification reaction of 28 S rRNA in eukaryotic ribosomes by ricin
A-chain was characterized. To examine whether ricin A-chain release any
bases from 28 S rRNA, rat liver ribosomes were incubated with a catalytic
amount of the toxin, and a fraction containing free bases and nucleosides
was prepared from the postribosomal fraction of the reaction mixture by
means of ion-exchange column chromatography. Thin- layer chromatographic
analysis of this fraction revealed a release of 1 mol of adenine from 1 mol
of ribosome. When the ribosomes or naked total RNAs were treated with ricin
A-chain in the presence of [32P] phosphate, little incorporation of the
radioactivity into 28 S rRNA was observed, indicating that the release is
not mediated by phosphorolysis. Thus, considering together with the
previous result (Endo, Y., Mitsui, K., Motizuki, M., and Tsurugi, K. (1987)
J. Biol. Chem. 262, 5908-5912), the results in the present experiments
demonstrated that ricin A-chain inactivates the ribosomes by cleaving the
N-glycosidic bond of A4324 of 28 S rRNA in a hydrolytic fashion.
RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes
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