| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 18, 8515-8521, 06, 1987
JS Davis, LL Weakland, RV Farese and LA West
The present studies were conducted to determine whether luteinizing hormone
(LH), a hormone which increases intracellular cAMP, also increases "second
messengers" derived from inositol phospholipid hydrolysis in isolated
bovine luteal cells. In luteal cells prelabeled with 32PO4, LH provoked
increases in labeling of phosphatidic acid, phosphatidylinositol, and
polyphosphatidylinositol (PIP). No reductions in 32P-prelabeled PIP and
PIP2 were observed in LH-treated cells. In luteal cells prelabeled with
myo-[2-3H]inositol, LH provoked rapid (10- 30 s) and sustained (up to 60
min) increases in the levels of inositol mono-, bis-, and trisphosphates
(IP, IP2, and IP3, respectively. IP3 was formed more rapidly than IP2 or IP
following LH treatment. In addition, LH increased (50%) levels of
[3H]inositol phospholipids in 30- min incubations. LiCl (10 mM) enhanced
inositol phosphate accumulation in response to LH. Maximal increases in IP3
occurred at 1-10 micrograms/ml of LH. Similar temporal and dose-response
relationships were observed for LH-stimulated IP3 and cAMP accumulation.
However, exogenous cAMP (8-bromo-cAMP, 5 mM) and forskolin (10 microM) had
no effect on inositol phosphate synthesis. The initial (1 min) effects of
LH on IP3 and cAMP were independent of extracellular calcium
concentrations, whereas the sustained (5 min) effect of LH on IP3, but not
cAMP, was dependent on a source of extracellular calcium. LH- stimulated
progesterone synthesis was also dependent on the presence of extracellular
calcium. LH induced rapid and concentration-dependent increases in [Ca2+]i
as measured by Quin 2 fluorescence. The LH-induced increases in [Ca2+]i
were maximal within 30 s (approximately 2-fold) and remained elevated for
at least 10 min. In Ca2+-free media containing 2 mM
[ethylenebis(oxyethylenenitrilo)]tetraacetic acid, LH was still able to
increase [Ca2+]i, but the increase was slightly less in magnitude and of
shorter duration (2-4 min). These findings demonstrate that LH can rapidly
raise levels of IP3 and [Ca2+]i, as well as, cAMP in bovine luteal cells.
These findings suggest that at least two second messenger systems exist to
mediate the action of LH in the corpus luteum.
Luteinizing hormone increases inositol trisphosphate and cytosolic free Ca2+ in isolated bovine luteal cells
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Tajima, A. Dantes, Z. Yao, K. Sorokina, F. Kotsuji, R. Seger, and A. Amsterdam Down-Regulation of Steroidogenic Response to Gonadotropins in Human and Rat Preovulatory Granulosa Cells Involves Mitogen-Activated Protein Kinase Activation and Modulation of DAX-1 and Steroidogenic Factor-1 J. Clin. Endocrinol. Metab., May 1, 2003; 88(5): 2288 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Salvador, E. Maizels, D. B. Hales, E. Miyamoto, H. Yamamoto, and M. Hunzicker-Dunn Acute Signaling by the LH Receptor Is Independent of Protein Kinase C Activation Endocrinology, August 1, 2002; 143(8): 2986 - 2994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Bebia, J. P. Somers, G. Liu, L. Ihrig, A. Shenker, and A. J. Zeleznik Adenovirus-Directed Expression of Functional Luteinizing Hormone (LH) Receptors in Undifferentiated Rat Granulosa Cells: Evidence for Differential Signaling through Follicle-Stimulating Hormone and LH Receptors Endocrinology, June 1, 2001; 142(6): 2252 - 2259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Aguirre, F. C. L. Jayes, and J. D. Veldhuis Luteinizing Hormone (LH) Drives Diverse Intracellular Calcium Second Messenger Signals in Isolated Porcine Ovarian Thecal Cells: Preferential Recruitment of Intracellular Ca2+ Oscillatory Cells by Higher Concentrations of LH Endocrinology, June 1, 2000; 141(6): 2220 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chakravorty, S. Mesiano, and R. B. Jaffe Corticotropin-Releasing Hormone Stimulates P450 17{alpha}-Hydroxylase/17,20-Lyase in Human Fetal Adrenal Cells via Protein Kinase C J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3732 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mamluk, N. Defer, J. Hanoune, and R. Meidan Molecular Identification of Adenylyl Cyclase 3 in Bovine Corpus Luteum and Its Regulation by Prostaglandin F2{alpha}-Induced Signaling Pathways Endocrinology, October 1, 1999; 140(10): 4601 - 4608. [Abstract] [Full Text]
|
|
|
|
|
|
A. Chamson-Reig, O. P. Pignataro, C. Libertun, and V. A. R. Lux-Lantos Alterations in Intracellular Messengers Mobilized by Gonadotropin-Releasing Hormone in an Experimental Ovarian Tumor Endocrinology, August 1, 1999; 140(8): 3573 - 3580. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Mukherjee, K. Palczewski, V. V. Gurevich, and M. Hunzicker-Dunn beta -Arrestin-dependent Desensitization of Luteinizing Hormone/Choriogonadotropin Receptor Is Prevented by a Synthetic Peptide Corresponding to the Third Intracellular Loop of the Receptor J. Biol. Chem., May 7, 1999; 274(19): 12984 - 12989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Rajagopalan-Gupta, S. Mukherjee, X. Zhu, Y.-K. Ho, H. Hamm, M. Birnbaumer, L. Birnbaumer, and M. Hunzicker-Dunn Roles of Gi and Gq/11 in Mediating Desensitization of the Luteinizing Hormone/Choriogonadotropin Receptor in Porcine Ovarian Follicular Membranes Endocrinology, April 1, 1999; 140(4): 1612 - 1621. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. O. Stocco and R. P. Deis Luteinizing Hormone Inhibits Conversion of Pregnenolone to Progesterone in Luteal Cells from Rats on Day 19 of Pregnancy Biol Reprod, March 1, 1999; 60(3): 729 - 732. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Rajagopalan-Gupta, M. L. G. Lamm, S. Mukherjee, M. M. Rasenick, and M. Hunzicker-Dunn Luteinizing Hormone/Choriogonadotropin Receptor-Mediated Activation of Heterotrimeric Guanine Nucleotide Binding Proteins in Ovarian Follicular Membranes Endocrinology, November 1, 1998; 139(11): 4547 - 4555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Flores, C. Aguirre, O. P. Sharma, and J. D. Veldhuis Luteinizing Hormone (LH) Stimulates Both Intracellular Calcium Ion ([Ca2+]i) Mobilization and Transmembrane Cation Influx in Single Ovarian (Granulosa) Cells: Recruitment as a Cellular Mechanism of LH-[Ca2+]i Dose Response Endocrinology, August 1, 1998; 139(8): 3606 - 3612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Rajagopalan-Gupta, M. M. Rasenick, and M. Hunzicker-Dunn Luteinizing Hormone/Choriogonadotropin-Dependent, Cholera Toxin-Catalyzed Adenosine 5'-Diphosphate (ADP)-Ribosylation of the Long and Short Forms of Gs{alpha} and Pertussis Toxin-Catalyzed ADP-Ribosylation of Gi{alpha} Mol. Endocrinol., May 1, 1997; 11(5): 538 - 549. [Abstract] [Full Text]
|
|
|
|
|
|
A. Herrlich, B. Kuhn, R. Grosse, A. Schmid, G. Schultz, and T. Gudermann Involvement of Gs and Gi Proteins in Dual Coupling of the Luteinizing Hormone Receptor to Adenylyl Cyclase and Phospholipase C J. Biol. Chem., July 12, 1996; 271(28): 16764 - 16772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mukherjee, J. E. Casanova, and M. Hunzicker-Dunn Desensitization of the Luteinizing Hormone/Choriogonadotropin Receptor in Ovarian Follicular Membranes Is Inhibited by Catalytically Inactive ARNO+ J. Biol. Chem., February 23, 2001; 276(9): 6524 - 6528. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
