| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 18, 8603-8609, Jun, 1987
H Sugino, GR Bousfield, WT Moore Jr and DN Ward
The complete amino acid sequence of the beta-subunit of equine chorionic
gonadotropin (eCG beta) has been established by both automated Edman and
manual 5-dimethylaminonaphthalene-1-sulfonyl-Edman degradations. Specific
fragments were produced by cleavage with Staphylococcus aureus V8 protease,
trypsin, or dilute HCl. For the sequence analyses of the heavily
glycosylated COOH-terminal portion, a chemical deglycosylation procedure
with trifluoromethanesulfonic acid was employed. The peptide chain of eCG
beta consists of 149 amino acid residues. Five or more oligosaccharide
chains are attached to the protein, 1 unit linked by an N-glycosidic bond
to asparagine at residue 13 and four or more units linked by O-glycosidic
bonds to serine or threonine at residues in the COOH-terminal portion. The
carbohydrate- bearing hydroxy amino acids have not yet been rigorously
established. As compared to the beta-subunits of the pituitary gonadotropin
hormones, lutropin, follitropin, and thyrotropin, eCG beta possesses a
glycosylated COOH-terminal extension of about 30 amino acid residues, as
does the human chorionic gonadotropin beta-subunit (hCG beta). When the
comparison is restricted inside the disulfide bond-containing core
(residues 1-110), the beta-subunit of eCG is highly homologous to hCG beta
(66%). On the other hand, although the overall structural features closely
resemble each other, much less homology exists in the COOH- terminal
extensions of eCG beta and hCG beta.
Structural studies on equine glycoprotein hormones. Amino acid sequence of equine chorionic gonadotropin beta-subunit
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Legardinier, D. Klett, J.-C. Poirier, Y. Combarnous, and C. Cahoreau Mammalian-like nonsialyl complex-type N-glycosylation of equine gonadotropins in MimicTM insect cells Glycobiology, August 1, 2005; 15(8): 776 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Saint-Dizier, F. Foulon-Gauze, F. Lecompte, Y. Combarnous, and M. Chopineau Cloning and functional expression of the equine luteinizing hormone/chorionic gonadotrophin receptor J. Endocrinol., December 1, 2004; 183(3): 551 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishigame, M. S. Medan, G. Watanabe, Z. Shi, H. Kishi, K. Y. Arai, and K. Taya A New Alternative Method for Superovulation Using Passive Immunization Against Inhibin in Adult Rats Biol Reprod, July 1, 2004; 71(1): 236 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Herve, F. Roy, J. Bertin, F. Guillou, and M.-C. Maurel Antiequine Chorionic Gonadotropin (eCG) Antibodies Generated in Goats Treated with eCG for the Induction of Ovulation Modulate the Luteinizing Hormone and Follicle-Stimulating Hormone Bioactivities of eCG Differently Endocrinology, January 1, 2004; 145(1): 294 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bousfield, V. Y. Butnev, and V. Y. Butnev Identification of Twelve O-Glycosylation Sites in Equine Chorionic Gonadotropin {beta} and Equine Luteinizing Hormone {beta} by Solid-Phase Edman Degradation Biol Reprod, January 1, 2001; 64(1): 136 - 147. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. W. Wolfe The Equine Luteinizing Hormone {beta}-Subunit Promoter Contains Two Functional Steroidogenic Factor-1 Response Elements Mol. Endocrinol., September 1, 1999; 13(9): 1497 - 1510. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
