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J. Biol. Chem., Vol. 262, Issue 21, 10035-10038, Jul, 1987
P Matsudaira
Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other
proteins and peptides can be spotted or electroblotted onto polyvinylidene
difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced
directly. The membranes are not chemically activated or pretreated with
Polybrene before usage. The average repetitive yields and initial coupling
of proteins spotted or blotted into PVDF membranes ranged between 84-98%
and 30-108% respectively, and were comparable with the yields measured for
proteins spotted onto Polybrene-coated glass fiber discs. The results
suggest that PVDF membranes are superior supports for sequence analysis of
picomole quantities of proteins purified by gel electrophoresis.
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
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