JBC Focus on PI3-Kinase with Echelon

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J. Biol. Chem., Vol. 262, Issue 21, 10044-10047, Jul, 1987

(2')3',5'-Bisphosphate nucleotidase

SG Ramaswamy and WB Jakoby

(2')3',5'-Bisphosphate nucleotidase has been prepared in electrophoretically homogeneous form from guinea pig liver. The enzyme catalyzes the hydrolysis of the 2'- or 3'-phosphate from the appropriate nucleoside 2',5'- and 3',5'-bisphosphates and is active with 3'-phosphoadenosine 5'-phosphosulfate and with coenzyme A but not with ATP. The 40,000-dalton protein is a monomer that requires Mg2+ for activity.
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