| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 23, 10971-10979, Aug, 1987
DP Cistola, DM Small and JA Hamilton
13C NMR chemical shift and intensity results for a series of carboxyl
13C-enriched saturated fatty acids (8-18 carbons) bound to bovine serum
albumin (BSA) are presented as a function of increasing fatty acid (FA)/BSA
mole ratio. Spectra for long-chain (greater than or equal to 12 carbons) FA
X BSA complexes exhibited up to five FA carboxyl resonances, designated a,
b, b', c, and d. Only three resonances (peaks b, b', and d) were observed
below 3:1 FA X BSA mole ratio, and at greater than or equal to 3:1 mole
ratio, two additional resonances were observed (peaks c and a). In a
spectrum of 5:1 stearic acid X BSA complexes, peaks b, b', and d each
represented approximately one-fifth, and peak c approximately two-fifths,
of the total FA carboxyl intensity. Plots of total carboxyl/carbonyl
intensity ratio as a function of FA X BSA mole ratio were linear up to 7-9
mole ratio. Deviation from linearity at mole ratios greater than or equal
to 7 was accompanied by the detection of crystalline unbound FA (as 1:1
acid/soap) by X-ray diffraction. In contrast to long-chain FA X BSA
complexes, 13C NMR spectra of octanoic acid X BSA complexes yielded only
one FA carboxyl resonance (peak c) at FA X BSA mole ratios between 1 and
20. We conclude: peaks b, b', and d represent FA bound to three individual
high affinity (primary) long-chain FA binding sites on BSA; peak c
represents FA bound to several secondary long-chain (or primary
short-chain) FA binding sites on BSA; peak a represents long-chain FA bound
to an additional lower affinity binding site. We present a model that
correlates the observed 13C NMR resonances with individual binding site
locations predicted by a recent three-dimensional model of BSA.
Carbon 13 NMR studies of saturated fatty acids bound to bovine serum albumin. I. The filling of individual fatty acid binding sites
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Lucke, D. L. Gantz, E. Klimtchuk, and J. A. Hamilton Interactions between fatty acids and {alpha}-synuclein J. Lipid Res., August 1, 2006; 47(8): 1714 - 1724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Feldkamp, A. Kribben, N. F. Roeser, R. A. Senter, and J. M. Weinberg Accumulation of nonesterified fatty acids causes the sustained energetic deficit in kidney proximal tubules after hypoxia-reoxygenation Am J Physiol Renal Physiol, February 1, 2006; 290(2): F465 - F477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Simard, P. A. Zunszain, C.-E. Ha, J. S. Yang, N. V. Bhagavan, I. Petitpas, S. Curry, and J. A. Hamilton Locating high-affinity fatty acid-binding sites on albumin by x-ray crystallography and NMR spectroscopy PNAS, December 13, 2005; 102(50): 17958 - 17963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Dansen, G. J. P. L. Kops, S. Denis, N. Jelluma, R. J. A. Wanders, J. L. Bos, B. M. T. Burgering, and K. W. A. Wirtz Regulation of sterol carrier protein gene expression by the Forkhead transcription factor FOXO3a J. Lipid Res., January 1, 2004; 45(1): 81 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. N. Bojesen and H. S. Hansen Binding of anandamide to bovine serum albumin J. Lipid Res., September 1, 2003; 44(9): 1790 - 1794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Guo, T. Lei, T. Wang, B. E. Corkey, and J. Han Octanoate Inhibits Triglyceride Synthesis in 3T3-L1 and Human Adipocytes J. Nutr., August 1, 2003; 133(8): 2512 - 2518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-K. Choi, J. Ho, S. Curry, D. Qin, R. Bittman, and J. A. Hamilton Interactions of very long-chain saturated fatty acids with serum albumin J. Lipid Res., July 1, 2002; 43(7): 1000 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Pound, J. X. Kang, and A. Leaf Partitioning of polyunsaturated fatty acids, which prevent cardiac arrhythmias, into phospholipid cell membranes J. Lipid Res., March 1, 2001; 42(3): 346 - 351. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Duff, S. Kalambur, and E. Boyle-Roden Serum Albumin Binds {beta}- and {{alpha}}-Monoolein In Vitro J. Nutr., March 1, 2001; 131(3): 774 - 778. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Caserta, T. Tchkonia, V. N. Civelek, M. Prentki, N. F. Brown, J. D. McGarry, R. A. Forse, B. E. Corkey, J. A. Hamilton, and J. L. Kirkland Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E238 - E247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. X Kang and A. Leaf Prevention of fatal cardiac arrhythmias by polyunsaturated fatty acids Am. J. Clinical Nutrition, January 1, 2000; 71(1): 202S - 207S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Billman, J. X. Kang, and A. Leaf Prevention of Sudden Cardiac Death by Dietary Pure {omega}-3 Polyunsaturated Fatty Acids in Dogs Circulation, May 11, 1999; 99(18): 2452 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Hamilton Fatty acid transport: difficult or easy? J. Lipid Res., March 1, 1998; 39(3): 467 - 481. [Abstract] [Full Text]
|
|
|
|
|
|
J. X. Kang and A. Leaf Antiarrhythmic Effects of Polyunsaturated Fatty Acids: Recent Studies Circulation, October 1, 1996; 94(7): 1774 - 1780. [Full Text]
|
|
|
|
 |
|
 P. Jezek, J. Hanus, C. Semrad, and K. D. Garlid Photoactivated Azido Fatty Acid Irreversibly Inhibits Anion and Proton Transport through the Mitochondrial Uncoupling Protein J. Biol. Chem., March 15, 1996; 271(11): 6199 - 6205. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
