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J. Biol. Chem., Vol. 262, Issue 23, 11057-11063, 08, 1987

In vitro methylation of the 5'-flanking regions of the mouse beta- globin gene

C Ward, A Bolden, CM Nalin and A Weissbach

The enzymatic methylation of the 5'-flanking region of the mouse beta- globin (major) gene containing putative regulatory regions has been investigated. In vitro methylation of this 368-base pair regulatory DNA by a DNA methyltransferase obtained from mouse erythroleukemia cells yields an asymmetric methylation pattern. Of the 10 available CG pairs, only 5-6 are modified, leading to one hemimethylated site and two apparently fully methylated sites. Only CG pairs which are localized in a 29-base pair cluster are methylated. The data suggest that a CG cluster approximately 100 base pairs upstream from the CAP site may be the in vivo site of methylation in the 5'-regulator region of the mouse beta-globin gene.
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