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J. Biol. Chem., Vol. 262, Issue 23, 11134-11139, Aug, 1987
AM Bonneau and N Sonenberg
The rate of protein synthesis in metaphase-arrested cells is reduced as
compared to interphase cells. The reduction occurs at the translation
initiation step. Here, we show that, whereas poliovirus RNA translation is
not affected by the mitotic translational block, the translation of
vesicular stomatitis virus mRNAs is. In an attempt to elucidate the
mechanism by which initiation of protein synthesis is reduced in mitotic
cells, we found that the interaction of the mRNA 24-kDa cap- binding
protein (CBP) with the mRNA 5' cap structure is reduced in mitotic cell
extracts, consistent with their lower translational efficiency. Addition of
cap-binding protein complex stimulated the translation of endogenous mRNA
in extracts from mitotic but not interphase cells. In addition, we found
that the 24-kDa CBP from mitotic cells was metabolically labeled with 32P
to a lesser extent than the protein purified from interphase cells. These
results are consistent with a hypothesis that the 24-kDa CBP is implicated
in the inhibition of protein synthesis in metaphase-arrested cells.
Possible mechanisms for this inhibition are offered.
Involvement of the 24-kDa cap-binding protein in regulation of protein synthesis in mitosis
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