| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 26, 12393-12396, Sep, 1987
J Hansen, T Schulze and K Moelling
The reverse transcriptase polymerase of the human T-cell lymphotropic
virus/lymphadenopathy-associated virus has been cloned into an expression
vector and expressed in Escherichia coli. Two polypeptides of 66 and 51 kDa
molecular mass are detectable in polymerase-expressing bacterial lysates
with human patient sera. They are processed from a short-lived 120-kDa
polyprotein precursor equivalent to a region consisting of polymerase,
protease, and endonuclease. The 51 kDa protein appears to originate from
the 66-kDa molecule; additional processing products are 32- and 15-kDa
proteins. The bacterially expressed polymerase is enzymatically active and
exhibits the template specificities, ion requirements, and response to
inhibitors of the authentic enzyme. It was purified by DEAE-cellulose-,
phosphocellulose- , and poly(rC)-agarose column chromatography followed by
glycerol density gradient centrifugation. It copurifies with an RNase H
activity, suggesting the existence of a virus-coded DNA polymerase- RNase H
complex. The purified bacterial enzyme allows a safe large- scale screening
for inhibitors of both activities.
RNase H activity associated with bacterially expressed reverse transcriptase of human T-cell lymphotropic virus III/lymphadenopathy- associated virus
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. L. White, N. A. Margot, T. Wrin, C. J. Petropoulos, M. D. Miller, and L. K. Naeger Molecular Mechanisms of Resistance to Human Immunodeficiency Virus Type 1 with Reverse Transcriptase Mutations K65R and K65R+M184V and Their Effects on Enzyme Function and Viral Replication Capacity Antimicrob. Agents Chemother., November 1, 2002; 46(11): 3437 - 3446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. F. Gerard, R. J. Potter, M. D. Smith, K. Rosenthal, G. Dhariwal, J. Lee, and Deb. K. Chatterjee The role of template-primer in protection of reverse transcriptase from thermal inactivation Nucleic Acids Res., July 15, 2002; 30(14): 3118 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Miller, P. D. Lamy, M. D. Fuller, A. S. Mulato, N. A. Margot, T. Cihlar, and J. M. Cherrington Human Immunodeficiency Virus Type 1 Reverse Transcriptase Expressing the K70E Mutation Exhibits a Decrease in Specific Activity and Processivity Mol. Pharmacol., August 1, 1998; 54(2): 291 - 297. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. P. Burnett and C. S. McHenry Posttranscriptional modification of retroviral primers is required for late stages of DNA replication PNAS, July 8, 1997; 94(14): 7210 - 7215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. DeStefano and J. J. DeStefano Interaction of Human Immunodeficiency Virus Nucleocapsid Protein with a Structure Mimicking a Replication Intermediate. EFFECTS ON STABILITY, REVERSE TRANSCRIPTASE BINDING, AND STRAND TRANSFER J. Biol. Chem., July 5, 1996; 271(27): 16350 - 16356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Sarafianos, V. N. Pandey, N. Kaushik, and M. J. Modak Site-directed Mutagenesis of Arginine 72 of HIV-1 Reverse Transcriptase J. Biol. Chem., August 25, 1995; 270(34): 19729 - 19735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, B. M. Blumberg, P. J. Fay, and R. A. Bambara Strand Transfer Mediated by Human Immunodeficiency Virus Reverse Transcriptase in Vitro Is Promoted by Pausing and Results in Misincorporation J. Biol. Chem., January 6, 1995; 270(1): 325 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. DeStefano, J. V. Cristofaro, S. Derebail, W. P. Bohlayer, and M. J. Fitzgerald-Heath Physical Mapping of HIV Reverse Transcriptase to the 5' End of RNA Primers J. Biol. Chem., August 24, 2001; 276(35): 32515 - 32521. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
