| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 29, 13953-13958, 10, 1987
DA Brow and EP Geiduschek
Department of Biology, University of California, San Diego, La Jolla 92093.
Synthesis of yeast 5 S rRNA in a homologous cell-free system has previously been shown to be inhibited by exogenous yeast 5 S rRNA. This inhibition is diminished when 5 S rDNA is first incubated with the cell- free system, implying the presence of a transcription factor IIIA analog in yeast, and is dependent on the nucleotide sequence of the 5 S rRNA added. A two-dimensional gel electrophoresis method was used to demonstrate assembly of newly synthesized 5 S rRNA into a ribonucleoprotein complex containing ribosomal protein YL3. Immunodepletion of the cell-free system with anti-YL3 IgG was found to increase the sensitivity of 5 S rRNA synthesis to inhibition by 5 S rRNA. We propose a model for the regulation of yeast 5 S rRNA synthesis in which YL3 competes with the presumptive transcription factor IIIA analog for binding of 5 S rRNA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. L. French, Y. N. Osheim, D. A. Schneider, M. L. Sikes, C. F. Fernandez, L. A. Copela, V. A. Misra, M. Nomura, S. L. Wolin, and A. L. Beyer Visual Analysis of the Yeast 5S rRNA Gene Transcriptome: Regulation and Role of La Protein Mol. Cell. Biol., July 15, 2008; 28(14): 4576 - 4587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Smith, A. Meskauskas, P. Wang, P. V. Sergiev, and J. D. Dinman Saturation Mutagenesis of 5S rRNA in Saccharomyces cerevisiae Mol. Cell. Biol., December 15, 2001; 21(24): 8264 - 8275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang and R. J. Maraia Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human Nucleic Acids Res., July 1, 2001; 29(13): 2675 - 2690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Pittman, M. T. Andrews, and D. R. Setzer A Feedback Loop Coupling 5 S rRNA Synthesis to Accumulation of a Ribosomal Protein J. Biol. Chem., November 19, 1999; 274(47): 33198 - 33201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Clarke, C. L. Peterson, A. V. Brainard, and D. L. Riggs Regulation of the RNA Polymerase I and III Transcription Systems in Response to Growth Conditions J. Biol. Chem., September 6, 1996; 271(36): 22189 - 22195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dieci, L. Duimio, G. Peracchia, and S. Ottonello Selective Inactivation of Two Components of the Multiprotein Transcription Factor TFIIIB in Cycloheximide Growth-arrested Yeast Cells J. Biol. Chem., June 2, 1995; 270(22): 13476 - 13482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Bordonne, J Banroques, J Abelson, and C Guthrie Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo. Genes & Dev., July 1, 1990; 4(7): 1185 - 1196. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
