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J. Biol. Chem., Vol. 262, Issue 31, 14879-14884, Nov, 1987
M Riviere, JJ Fournie and G Puzo
Using high-performance liquid chromatography, a new kind of phenolic
glycolipid quantitatively minor, called phenolic glycolipid-II, was
isolated from a lipidic fraction of Mycobacterium kansasii. The structure
was determined by fast atom bombardment-mass spectrometry and proton
nuclear magnetic resonance spectroscopy, as: 2,4-di-O-Me-alpha-D-
Manp(1----3) 4-O-Ac-2-O-Me-alpha-L-Fucp(1----3)2-O-Me- alpha-L-Rhap(1---
-3) 2,4-di-O-Me-alpha-L-Rhap 1----phenolphthiocerol dimycocerosate.
Phenolic glycolipids I and II differ only by their distal monosaccharide
hapten which is 2,6-dideoxy-4-O-Me-alpha-D- arabinohexopyranosyl and the
2,4-di-O-Me-alpha-D-mannopyranosyl, respectively. This sugar appears to be
characteristic and apparently unique in the Mycobacterium genus. Moreover,
phenolic glycolipids I and II constitute with the lipooligosaccharides two
classes of antigens of M. kansasii.
A novel mannose containing phenolic glycolipid from Mycobacterium kansasii
Centre de Recherche de Biochimie et de Genetique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
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