| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 34, 16275-16278, Dec, 1987
R Horuk, JJ Huang, M Covington and RC Newton
Medical Products Division, E. I. Du Pont De Nemours & Co., Glenolden Laboratory, Glenolden, Pennsylvania 19036.
This study describes the biochemical characterization and kinetic analysis of the interleukin-1 (IL-1) receptor in Raji human B-lymphoma and EL4 murine T-lymphoma cells. The internalization of 125I-IL-1 was studied in both cell types by an acid extraction technique which removes surface bound ligand. At 37 degrees C, binding to Raji IL-1 receptors was almost entirely cell surface (91%). EL4 cells, in contrast, internalized 59% of ligand at this temperature and this was almost totally inhibited by sodium azide. Receptor binding studies showed that the B-cells had a lower binding affinity but much higher receptor density per cell (KD = 2.1 nM, Ro = 7709) than the T-cells (KD = 0.4 nM, Ro = 241). The receptor binding affinity of two IL-1 analogs, Glu-4 and clone 18, was determined in competitive binding studies. In the B-cells the analogs had binding affinities of 25 and 90%, respectively, whereas in the T-cells the affinities were 0.2 and 200%, respectively. Chemical cross-linking studies showed that the IL-1 receptor in B-cells had a lower molecular weight than that in T-cells (68 kDa compared to 80 kDa). In summary these studies demonstrate that structural differences exist between IL-1 receptors in Raji and EL4 cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Bourke, A. Cassetti, A. Villa, E. Fadlon, F. Colotta, and A. Mantovani IL-1{beta} Scavenging by the Type II IL-1 Decoy Receptor in Human Neutrophils J. Immunol., June 15, 2003; 170(12): 5999 - 6005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Kozak, S. E. Kuhmann, E. J. Platt, and D. Kabat Roles of CD4 and Coreceptors in Binding, Endocytosis, and Proteolysis of gp120 Envelope Glycoproteins Derived from Human Immunodeficiency Virus Type 1 J. Biol. Chem., August 13, 1999; 274(33): 23499 - 23507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Valles, C. Tsoi, W.-Y. Huang, D. Wyllie, F. Carlotti, J. A. Askari, M. J. Humphries, S. K. Dower, and E. E. Qwarnstrom Recruitment of a Heparan Sulfate Subunit to the Interleukin-1 Receptor Complex. REGULATION BY FIBRONECTIN ATTACHMENT J. Biol. Chem., July 16, 1999; 274(29): 20103 - 20109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Platt, K. Wehrly, S. E. Kuhmann, B. Chesebro, and D. Kabat Effects of CCR5 and CD4 Cell Surface Concentrations on Infections by Macrophagetropic Isolates of Human Immunodeficiency Virus Type 1 J. Virol., April 1, 1998; 72(4): 2855 - 2864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Greenfeder, P. Nunes, L. Kwee, M. Labow, R. A. Chizzonite, and G. Ju Molecular Cloning and Characterization of a Second Subunit of the Interleukin 1 Receptor Complex J. Biol. Chem., June 9, 1995; 270(23): 13757 - 13765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Colotta, F Re, M Muzio, R Bertini, N Polentarutti, M Sironi, J. Giri, S. Dower, J. Sims, and A Mantovani Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4 Science, July 23, 1993; 261(5120): 472 - 475. [Abstract] [PDF]
|
|
|
|
|
|
J. Sims, C. March, D Cosman, M. Widmer, H. MacDonald, C. McMahan, C. Grubin, J. Wignall, J. Jackson, S. Call, et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily Science, July 29, 1988; 241(4865): 585 - 589. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
