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J. Biol. Chem., Vol. 262, Issue 34, 16289-16293, Dec, 1987

Complete nucleotide sequence of reovirus L2 gene and deduced amino acid sequence of viral mRNA guanylyltransferase

LS Seliger, K Zheng and AJ Shatkin
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08855-0759.

Reovirus mRNAs synthesized by the virion-associated RNA polymerase contain a 5'-terminal cap that is added to nascent transcripts by polypeptide lambda 2, a structural component of virions encoded by double-stranded RNA genome segment L2. The complete, 3916-nucleotide sequence of a full-length reovirus type 3 L2 DNA clone was determined by the dideoxy chain terminator method. The sequence has a single long open reading frame extending from the second A-T-G at nucleotide 14 to a termination codon at position 3881. On this basis, the 1289-amino acid sequence of polypeptide lambda 2, the reovirus mRNA guanylyltransferase, was deduced and compared to other GTP-binding proteins. Two different, lysine-containing lambda 2 peptide sequences closely resemble predicted amino acid stretches in vaccinia virus guanylyltransferase and potentially form part of active sites in the viral mRNA capping enzymes.
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