| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 34, 16536-16545, 12, 1987
KE Kropp, J Gulick and J Robbins
Recently we have isolated a large number of chicken myosin or myosin- like
heavy chain genes. Seven of these genes were placed into a subset based
upon their hybridization patterns. In the present study, the sequence of
the 5' end of one of the myosin heavy chain (MHC) genes, N127, was
determined and compared with the 5' end sequences of the other six MHC
genes in the subset. The comparison revealed that the three exons encoding
the amino termini of the protein are highly conserved. The sequence
analysis shows that a localized correction event occurred in and around a
domain of the nucleotide-binding site, as the exon encoding this site and
the preceding intron are very highly conserved among the seven genes. The
sequence of the promoter and 5'- untranslated region of N127 is presented.
The analogous regions for N124 and N125 have now been sequenced and are
also presented. As is the case for all the other known MHC genes, the
5'-untranslated regions are split by large introns. The promoter and
5'-untranslated regions are compared with two previously characterized
chicken MHC genes (N116 and N118) to determine the sequence similarities
and differences that might underlie the differential expression of the
family's members. To confirm and extend previously published results of the
expression of these genes, transcript-specific probes generated from the 5'
region of six of the seven genes were used to determine in which muscle(s)
the corresponding mRNAs were present. The data show that despite the very
close structural homologies, each of the genes for which a unique probe
could be prepared exhibits a unique pattern of expression.
Structural and transcriptional analysis of a chicken myosin heavy chain gene subset
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. M. Kier and F. H. Schachat Muscle specialization in the squid motor system J. Exp. Biol., January 15, 2008; 211(2): 164 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, C. L. Moncman, and D. A. Winkelmann Mutations in the motor domain modulate myosin activity and myofibril organization J. Cell Sci., October 15, 2003; 116(20): 4227 - 4238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Lakich, T. T. Diagana, D. L. North, and R. G. Whalen MEF-2 and Oct-1 Bind to Two Homologous Promoter Sequence Elements and Participate in the Expression of a Skeletal Muscle-specific Gene J. Biol. Chem., June 12, 1998; 273(24): 15217 - 15226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Nikovits Jr., G. F. Wang, J. L. Feldman, J. B. Miller, R. Wade, L. Nelson, and F. E. Stockdale Isolation and Characterization of an Avian Slow Myosin Heavy Chain Gene Expressed during Embryonic Skeletal Muscle Fiber Formation J. Biol. Chem., July 19, 1996; 271(29): 17047 - 17056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.'i. Takeda, D. L. North, T. Diagana, Y. Miyagoe, M. M. Lakich, and R. G. Whalen Myogenic Regulatory Factors Can Activate TATA-containing Promoter Elements via an E-Box Independent Mechanism J. Biol. Chem., June 30, 1995; 270(26): 15664 - 15670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chang, K Fernandes, and M. Dauncey Molecular characterization of a developmentally regulated porcine skeletal myosin heavy chain gene and its 5' regulatory region J. Cell Sci., January 4, 1995; 108(4): 1779 - 1789. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
