| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 36, 17443-17449, Dec, 1987
IR van Driel, JL Goldstein, TC Sudhof and MS Brown
The ligand binding domain of the low density lipoprotein receptor consists
of seven cysteine-rich repeats of approximately 40 amino acids each. These
repeats, which are located at the NH2 terminus of the protein, are
homologous to sequences in complement components C8 and C9. To determine
the role of the first repeat (amino acids 2-42), we prepared two plasmids
containing expressible low density lipoprotein receptor cDNAs. The first
plasmid, p delta R1, lacks only the nucleotides encoding the first repeat.
It produced a receptor that bound and internalized lipoproteins and
recycled to the cell surface with the same efficiency as the normal
receptor. This deleted receptor failed to bind two monoclonal antibodies,
IgG-C7 and IgG-15C8, which were shown previously to react with the
ligand-binding domain. The second plasmid, pR1, encodes a markedly
truncated protein whose extracellular domain consists of the first repeat
joined to the transmembrane and cytoplasmic domains. This protein bound the
two monoclonal antibodies with the same affinity as the normal receptor,
but failed to bind lipoproteins. Binding of IgG-15C8 to the normal receptor
and the pR1-encoded protein was Ca2+-dependent, indicating that the first
repeat binds Ca2+. We conclude that repeats 2-6 in the ligand-binding
domain are sufficient for binding lipoproteins and that the first repeat is
highly immunogenic, but is not required for lipoprotein binding.
First cysteine-rich repeat in ligand-binding domain of low density lipoprotein receptor binds Ca2+ and monoclonal antibodies, but not lipoproteins
Department of Molecular Genetics, University of Texas Health Science Center, Southwestern Medical School, Dallas 75235.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. E. Infante, L. Abi-Mosleh, A. Radhakrishnan, J. D. Dale, M. S. Brown, and J. L. Goldstein Purified NPC1 Protein: I. BINDING OF CHOLESTEROL AND OXYSTEROLS TO A 1278-AMINO ACID MEMBRANE PROTEIN J. Biol. Chem., January 11, 2008; 283(2): 1052 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Infante, A. Radhakrishnan, L. Abi-Mosleh, L. N. Kinch, M. L. Wang, N. V. Grishin, J. L. Goldstein, and M. S. Brown Purified NPC1 Protein: II. LOCALIZATION OF STEROL BINDING TO A 240-AMINO ACID SOLUBLE LUMINAL LOOP J. Biol. Chem., January 11, 2008; 283(2): 1064 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Hopkins, S. Layfield, T. Ferraro, R. A. D. Bathgate, and P. R. Gooley The NMR Solution Structure of the Relaxin (RXFP1) Receptor Lipoprotein Receptor Class A Module and Identification of Key Residues in the N-terminal Region of the Module That Mediate Receptor Activation J. Biol. Chem., February 9, 2007; 282(6): 4172 - 4184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Nguyen, T. Hirama, V. Chauhan, R. MacKenzie, and R. Milne Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr J. Lipid Res., July 1, 2006; 47(7): 1399 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Herdy, L. Snyers, M. Reithmayer, P. Hinterdorfer, and D. Blaas Identification of the Human Rhinovirus Serotype 1A Binding Site on the Murine Low-Density Lipoprotein Receptor by Using Human-Mouse Receptor Chimeras J. Virol., July 1, 2004; 78(13): 6766 - 6774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Verges, A. Bensadoun, J. Herz, J. D. Belcher, and R. J. Havel Endocytosis of Hepatic Lipase and Lipoprotein Lipase into Rat Liver Hepatocytes in Vivo Is Mediated by the Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., March 5, 2004; 279(10): 9030 - 9036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Watanabe, T. Shimada, M. Kuroyanagi, M. Nishimura, and I. Hara-Nishimura Calcium-mediated Association of a Putative Vacuolar Sorting Receptor PV72 with a Propeptide of 2S Albumin J. Biol. Chem., March 1, 2002; 277(10): 8708 - 8715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wünschmann, J. D. Medh, D. Klinzmann, W. N. Schmidt, and J. T. Stapleton Characterization of Hepatitis C Virus (HCV) and HCV E2 Interactions with CD81 and the Low-Density Lipoprotein Receptor J. Virol., November 1, 2000; 74(21): 10055 - 10062. [Abstract] [Full Text]
|
|
|
|
|
|
O. K. Rødningen, S. Tonstad, J. D. Medh, D. A. Chappell, L. Ose, and T. P. Leren Phenotypic consequences of a deletion of exons 2 and 3 of the LDL receptor gene J. Lipid Res., February 1, 1999; 40(2): 213 - 220. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Obermoeller, Z. Chen, A. L. Schwartz, and G. Bu Ca2+ and Receptor-associated Protein Are Independently Required for Proper Folding and Disulfide Bond Formation of the Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., August 28, 1998; 273(35): 22374 - 22381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Dirlam-Schatz and A. D. Attie Calcium induces a conformational change in the ligand binding domain of the low density lipoprotein receptor J. Lipid Res., February 1, 1998; 39(2): 402 - 411. [Abstract] [Full Text]
|
|
|
|
|
|
T. Simmons, Y. M. Newhouse, K. S. Arnold, T. L. Innerarity, and K. H. Weisgraber Human Low Density Lipoprotein Receptor Fragment. SUCCESSFUL REFOLDING OF A FUNCTIONALLY ACTIVE LIGAND-BINDING DOMAIN PRODUCED IN ESCHERICHIA COLI J. Biol. Chem., October 10, 1997; 272(41): 25531 - 25536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Medh, S. L. Bowen, G. L. Fry, S. Ruben, M. Andracki, I. Inoue, J.-M. Lalouel, D. K. Strickland, and D. A. Chappell Lipoprotein Lipase Binds to Low Density Lipoprotein Receptors and Induces Receptor-mediated Catabolism of Very Low Density Lipoproteins in Vitro J. Biol. Chem., July 19, 1996; 271(29): 17073 - 17080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sironi, L. Mussoni, L. Prati, D. Baldassarre, M. Camera, C. Banfi, and E. Tremoli Plasminogen Activator Inhibitor Type-1 Synthesis and mRNA Expression in HepG2 Cells Are Regulated by VLDL Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 89 - 96. [Abstract] [Full Text]
|
|
|
|
|
|
C. Sass, L.-M. Giroux, S. Lussier-Cacan, J. Davignon, and A. Minnich Unexpected Consequences of Deletion of the First Two Repeats of the Ligand-binding Domain from the Low Density Lipoprotein Receptor J. Biol. Chem., October 20, 1995; 270(42): 25166 - 25171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Medh, G. L. Fry, S. L. Bowen, M. W. Pladet, D. K. Strickland, and D. A. Chappell The 39-kDa Receptor-associated Protein Modulates Lipoprotein Catabolism by Binding to LDL Receptors J. Biol. Chem., January 13, 1995; 270(2): 536 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gotthardt, M. Trommsdorff, M. F. Nevitt, J. Shelton, J. A. Richardson, W. Stockinger, J. Nimpf, and J. Herz Interactions of the Low Density Lipoprotein Receptor Gene Family with Cytosolic Adaptor and Scaffold Proteins Suggest Diverse Biological Functions in Cellular Communication and Signal Transduction J. Biol. Chem., August 11, 2000; 275(33): 25616 - 25624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jeon and G. G. Shipley Localization of the N-terminal Domain of the Low Density Lipoprotein Receptor J. Biol. Chem., September 22, 2000; 275(39): 30465 - 30470. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
