HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Epand, R. M. Articles by McKenzie, R. C. Search for Related Content PubMed PubMed Citation Articles by Epand, R. M. Articles by McKenzie, R. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 4, 1526-1529, 02, 1987

Effects of viral chemotherapeutic agents on membrane properties. Studies of cyclosporin A, benzyloxycarbonyl-D-Phe-L-Phe-Gly and amantadine

RM Epand, RF Epand and RC McKenzie

Cyclosporin A, benzyloxycarbonyl-D-Phe-L-Phe-Gly, and amantadine inhibit the dilution of fluorescently labeled lipids, as measured with the resonance energy exchange assay for membrane fusion. The fusion was studied using sonicated vesicles containing 1,2-dioleoyl-sn- glycero(3)phosphoethanolamine, egg (3-sn-phosphatidyl)choline, and cholesterol in a 1:1:1.3 molar ratio. All three antiviral agents inhibited myelin basic protein-induced membrane fusion when present at low concentrations in the membrane. The mechanism by which these agents affect membrane properties was investigated. The effect of these agents on the bilayer to hexagonal phase transition of 1,2-dielaidoyl-sn- glycero(3)phosphoethanolamine was determined using both differential scanning calorimetry and 31P NMR. Benzyloxycarbonyl-D-Phe-L-Phe-Gly is particularly effective in raising the bilayer to hexagonal phase transition temperature while cyclosporin promotes the greatest amount of broadening of the 31P NMR signal. Both effects are suggested to be related to the inhibitory activity of these substances on membrane fusion and possibly also to their antiviral activity.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. Jing, C. Ma, Y. Ohigashi, F. A. Oliveira, T. S. Jardetzky, L. H. Pinto, and R. A. Lamb Functional studies indicate amantadine binds to the pore of the influenza A virus M2 proton-selective ion channel PNAS, August 5, 2008; 105(31): 10967 - 10972. [Abstract] [Full Text] [PDF]

				C. Li, M. Yi, J. Hu, H.-X. Zhou, and T. A. Cross Solid-State NMR and MD Simulations of the Antiviral Drug Amantadine Solubilized in DMPC Bilayers Biophys. J., February 15, 2008; 94(4): 1295 - 1302. [Abstract] [Full Text] [PDF]

				S. Lucken-Ardjomande and J.-C. Martinou Newcomers in the process of mitochondrial permeabilization J. Cell Sci., February 1, 2005; 118(3): 473 - 483. [Abstract] [Full Text] [PDF]

				R. F. Epand, J.-C. Martinou, M. Fornallaz-Mulhauser, D. W. Hughes, and R. M. Epand The Apoptotic Protein tBid Promotes Leakage by Altering Membrane Curvature J. Biol. Chem., August 30, 2002; 277(36): 32632 - 32639. [Abstract] [Full Text] [PDF]

				T. Söderlund, J. Y. A. Lehtonen, and P. K. J. Kinnunen Interactions of Cyclosporin A with Phospholipid Membranes: Effect of Cholesterol Mol. Pharmacol., January 1, 1999; 55(1): 32 - 38. [Abstract] [Full Text]

				M. Vidal and D. Hoekstra In Vitro Fusion of Reticulocyte Endocytic Vesicles with Liposomes J. Biol. Chem., July 28, 1995; 270(30): 17823 - 17829. [Abstract] [Full Text] [PDF]