HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van der Valk, J. Articles by Moolenaar, W. H. Search for Related Content PubMed PubMed Citation Articles by van der Valk, J. Articles by Moolenaar, W. H. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 6, 2431-2434, Feb, 1987

Expression of pp60v-src alters the ionic permeability of the plasma membrane in rat cells

J van der Valk, I Verlaan, SW de Laat and WH Moolenaar

The transmembrane potential of Rous sarcoma virus (RSV)-infected Rat-1 cells, expressing the pp60v-src protein kinase, is markedly less negative (by approximately 30 mV) than that of their normal counterparts. By contrast, the membrane potential of Rat-1 cells infected with Kirsten sarcoma virus is virtually unaltered. The RSV- induced membrane depolarization is shown to be due to a severalfold increase in the cation permeability ratio (PNa/PK) of the plasma membrane. When cells infected with a temperature-sensitive mutant of RSV (ts LA29), encoding a src protein with heat-labile kinase activity, are shifted from the nonpermissive to the permissive temperature, a rapid and sustained membrane depolarization is observed. Conversely, thermal inactivation of the ts LA29 pp60v-src kinase activity rapidly restores the membrane potential to near normal levels. Addition of epidermal growth factor, platelet-derived growth factor, or insulin to uninfected cells fails to cause a detectable change in membrane potential. We conclude that, unlike growth factor receptor tyrosine kinases, pp60v-src can induce, either directly or indirectly, a major change in the membrane permeability to monovalent cations.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M. Summy, Y. Qian, B.-H. Jiang, A. Guappone-Koay, A. Gatesman, X. Shi, and D. C. Flynn The SH4-Unique-SH3-SH2 domains dictate specificity in signaling that differentiate c-Yes from c-Src J. Cell Sci., June 15, 2003; 116(12): 2585 - 2598. [Abstract] [Full Text] [PDF]

				F. R. Postma, T. Hengeveld, J. Alblas, B. N.G. Giepmans, G. C.M. Zondag, K. Jalink, and W. H. Moolenaar Acute loss of Cell-Cell Communication Caused by G Protein-coupled Receptors: A Critical Role for c-Src J. Cell Biol., March 9, 1998; 140(5): 1199 - 1209. [Abstract] [Full Text] [PDF]

				A Veithen, P Cupers, P Baudhuin, and P. Courtoy v-Src induces constitutive macropinocytosis in rat fibroblasts J. Cell Sci., January 8, 1996; 109(8): 2005 - 2012. [Abstract] [PDF]