HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rao, K. R. Articles by Tarr, G. E. Search for Related Content PubMed PubMed Citation Articles by Rao, K. R. Articles by Tarr, G. E. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 6, 2672-2675, Feb, 1987

Primary structure of an analog of crustacean pigment-dispersing hormone from the lubber grasshopper Romalea microptera

KR Rao, CJ Mohrherr, JP Riehm, CA Zahnow, S Norton, L Johnson and GE Tarr

An octadecapeptide capable of inducing pigment dispersion in the chromatophores of the fiddler crab Uca pugilator has been isolated from lyophilized heads of the lubber grasshopper Romalea microptera. This pigment-dispersing factor (PDF) was purified by gel filtration, ion- exchange chromatography, partition chromatography, and reversed-phase high performance liquid chromatography. Automated gas-phase sequencing, followed by the identification of the carboxyl-terminal amide, established the primary structure of this PDF as Asn-Ser-Glu-Ile-Ile- Asn-Ser-Leu-Leu-Gly-Leu-Pro-Lys-Leu-Leu-Asn-Asp-Ala- NH2. This structure was confirmed by chemical synthesis and by demonstrating that the synthetic and native PDF displayed identical chromatographic behavior and biological activity. The Romalea PDF is structurally related to the crustacean pigment-dispersing hormones (PDHs), which are also octadecapeptides. The sequence of grasshopper PDF shows 78% homology with beta-PDH (from the crabs U. pugilator and Cancer magister) and 50% homology with alpha-PDH (from the prawn Pandalus borealis). This study provides the first direct chemical evidence for the structural relatedness of insect PDF to the crustacean PDHs, thus identifying them as an authentic family of arthropod peptides.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Matsushima, K. Takano, T. Yoshida, Y. Takeda, S. Yokotani, Y. Shimohigashi, and M. Shimohigashi Double-labelled in situ Hybridization Reveals the Lack of Co-localization of mRNAs for the Circadian Neuropeptide PDF and FMRFamide in Brains of the Flies Musca domestica and Drosophila melanogaster J. Biochem., June 1, 2007; 141(6): 867 - 877. [Abstract] [Full Text] [PDF]

				E. Kula, E. S. Levitan, E. Pyza, and M. Rosbash PDF Cycling in the Dorsal Protocerebrum of the Drosophila Brain Is Not Necessary for Circadian Clock Function. J Biol Rhythms, April 1, 2006; 21(2): 104 - 117. [Abstract] [PDF]

				K. R. Rao Crustacean Pigmentary-Effector Hormones: Chemistry and Functions of RPCH, PDH, and Related Peptides Integr. Comp. Biol., June 1, 2001; 41(3): 364 - 379. [Abstract] [Full Text] [PDF]