| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 7, 2957-2967, 03, 1987
JA McDonald, BJ Quade, TJ Broekelmann, R LaChance, K Forsman, E Hasegawa and S Akiyama
Fibroblasts organize the modular cell-adhesive glycoprotein fibronectin
into a highly structured pericellular matrix by poorly understood
mechanisms. Previous studies implicated an amino-terminal domain in matrix
assembly and suggested that fibronectin's cell-adhesive domain and the
corresponding fibroblast receptor were not involved in this process. To
further elucidate the fibronectin region(s) involved in matrix assembly, we
mapped a library of proteolytic fragments and antibodies to various
fibronectin domains. The fragments and antibodies were used to probe the
role of fibronectin's amino-terminal and cell- adhesive domains in a
fibroblast matrix assembly assay. We found that fibronectin fragments
including the first 25-kDa sequence of fibronectin and antibodies to
amino-terminal domains inhibited pericellular matrix assembly. Polyclonal
antibodies to the 40-kDa collagen binding domain following the 25-kDa
amino-terminal domain also inhibited matrix assembly. However, collagen
binding is not required for matrix assembly as neither monoclonals blocking
collagen binding nor purified collagen binding domains themselves inhibited
matrix assembly. Therefore, the amino-terminal region of fibronectin
contains a site important in matrix assembly, and most activity is present
in the first 25-kDa of fibronectin. Fibronectin's cell-adhesive domain and
the fibroblast receptor binding to this domain also play an important role
in fibronectin matrix assembly. Apart from a monoclonal antibody to the
amino-terminal domain, only monoclonal antibodies binding to fibronectin's
cell-adhesive domain and inhibiting cell adhesion also inhibited matrix
assembly. In addition a 105-kDa fragment containing the cell-adhesive
domain inhibited matrix assembly. We conclude that at least two discrete
and widely separated sites in fibronectin with different binding
properties--the carboxyl-terminal fibroblast cell- adhesive domain and an
amino-terminal matrix assembly domain localized primarily within the first
25 kDa--are required for fibronectin pericellular matrix assembly by
fibroblasts. Fibronectin's cell- adhesive domain and its cell
surface-receptor complex appear to be involved in the matrix assembly
process prior to a step involving the amino-terminal domain. We believe
that this step is likely to be the initiation of cell-associated
fibronectin fibril formation by the fibronectin-adhesive-receptor complex.
Fibronectin's cell-adhesive domain and an amino-terminal matrix assembly domain participate in its assembly into fibroblast pericellular matrix
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Takahashi, M. Leiss, M. Moser, T. Ohashi, T. Kitao, D. Heckmann, A. Pfeifer, H. Kessler, J. Takagi, H. P. Erickson, et al. The RGD motif in fibronectin is essential for development but dispensable for fibril assembly J. Cell Biol., October 3, 2007; 178(1): 167 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Somanath, E. S. Kandel, N. Hay, and T. V. Byzova Akt1 Signaling Regulates Integrin Activation, Matrix Recognition, and Fibronectin Assembly J. Biol. Chem., August 3, 2007; 282(31): 22964 - 22976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yoneda, D. Ushakov, H. A.B. Multhaupt, and J. R. Couchman Fibronectin Matrix Assembly Requires Distinct Contributions from Rho Kinases I and -II Mol. Biol. Cell, January 1, 2007; 18(1): 66 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Mao and J. E. Schwarzbauer Stimulatory effects of a three-dimensional microenvironment on cell-mediated fibronectin fibrillogenesis J. Cell Sci., October 1, 2005; 118(19): 4427 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wang, R. A. F. Clark, D. F. Mosher, and X.-D. Ren Fibronectin's Central Cell-binding Domain Supports Focal Adhesion Formation and Rho Signal Transduction J. Biol. Chem., August 5, 2005; 280(31): 28803 - 28810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bae, T. Sakai, and D. F. Mosher Assembly of Exogenous Fibronectin by Fibronectin-null Cells Is Dependent on the Adhesive Substrate J. Biol. Chem., August 20, 2004; 279(34): 35749 - 35759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Knowles, F. Axelrod, C. D. Browne, and J. W. Smith A Fatty Acid Synthase Blockade Induces Tumor Cell-cycle Arrest by Down-regulating Skp2 J. Biol. Chem., July 16, 2004; 279(29): 30540 - 30545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Monaghan, V. Gueorguiev, C. Wilkins-Port, and P. J. McKeown-Longo The Receptor for Urokinase-type Plasminogen Activator Regulates Fibronectin Matrix Assembly in Human Skin Fibroblasts J. Biol. Chem., January 9, 2004; 279(2): 1400 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Wierzbicka-Patynowski and J. E. Schwarzbauer The ins and outs of fibronectin matrix assembly J. Cell Sci., August 15, 2003; 116(16): 3269 - 3276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Colombi, N. Zoppi, G. De Petro, E. Marchina, R. Gardella, D. Tavian, S. Ferraboli, and S. Barlati Matrix Assembly Induction and Cell Migration and Invasion Inhibition by a 13-Amino Acid Fibronectin Peptide J. Biol. Chem., April 11, 2003; 278(16): 14346 - 14355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Rotundo, T. M. Curtis, M. D. Shah, B. Gao, A. Mastrangelo, S. E. LaFlamme, and T. M. Saba TNF-alpha disruption of lung endothelial integrity: reduced integrin mediated adhesion to fibronectin Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L316 - L329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix J. Cell Sci., January 2, 2002; 115(3): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Sechler, H. Rao, A. M. Cumiskey, I. Vega-Colon, M. S. Smith, T. Murata, and J. E. Schwarzbauer A novel fibronectin binding site required for fibronectin fibril growth during matrix assembly J. Cell Biol., September 3, 2001; 154(5): 1081 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E. Olorundare, O. Peyruchaud, R. M. Albrecht, and D. F. Mosher Assembly of a fibronectin matrix by adherent platelets stimulated by lysophosphatidic acid and other agonists Blood, July 1, 2001; 98(1): 117 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pankov, E. Cukierman, B.-Z. Katz, K. Matsumoto, D. C. Lin, S. Lin, C. Hahn, and K. M. Yamada Integrin Dynamics and Matrix Assembly: Tensin-dependent Translocation of {alpha}5{beta}1 Integrins Promotes Early Fibronectin Fibrillogenesis J. Cell Biol., March 6, 2000; 148(5): 1075 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sechler, A. Cumiskey, D. Gazzola, and J. Schwarzbauer A novel RGD-independent fibronectin assembly pathway initiated by alpha4beta1 integrin binding to the alternatively spliced V region J. Cell Sci., January 4, 2000; 113(8): 1491 - 1498. [Abstract] [PDF]
|
|
|
|
|
|
C. Klass, J. Couchman, and A Woods Control of extracellular matrix assembly by syndecan-2 proteoglycan J. Cell Sci., January 2, 2000; 113(3): 493 - 506. [Abstract] [PDF]
|
|
|
|
|
|
Q. Zhang, T. Sakai, J. Nowlen, I. Hayashi, R. Fassler, and D. F. Mosher Functional beta 1-Integrins Release the Suppression of Fibronectin Matrix Assembly by Vitronectin J. Biol. Chem., January 1, 1999; 274(1): 368 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Sechler and J. E. Schwarzbauer Control of Cell Cycle Progression by Fibronectin Matrix Architecture J. Biol. Chem., October 2, 1998; 273(40): 25533 - 25536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhong, M. Chrzanowska-Wodnicka, J. Brown, A. Shaub, A. M. Belkin, and K. Burridge Rho-mediated Contractility Exposes a Cryptic Site in Fibronectin and Induces Fibronectin Matrix Assembly J. Cell Biol., April 20, 1998; 141(2): 539 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. O. Mercurius and A. O. Morla Inhibition of Vascular Smooth Muscle Cell Growth by Inhibition of Fibronectin Matrix Assembly Circ. Res., March 23, 1998; 82(5): 548 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bultmann, A. J. Santas, and D. M. P. Peters Fibronectin Fibrillogenesis Involves the Heparin II Binding Domain of Fibronectin J. Biol. Chem., January 30, 1998; 273(5): 2601 - 2609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wu, S. Y. Keightley, C. Leung-Hagesteijn, G. Radeva, M. Coppolino, S. Goicoechea, J. A. McDonald, and S. Dedhar Integrin-linked Protein Kinase Regulates Fibronectin Matrix Assembly, E-cadherin Expression, and Tumorigenicity J. Biol. Chem., January 2, 1998; 273(1): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Christopher, A. Kowalczyk, and P. McKeown-Longo Localization of fibronectin matrix assembly sites on fibroblasts and endothelial cells J. Cell Sci., January 3, 1997; 110(5): 569 - 581. [Abstract] [PDF]
|
|
|
|
|
|
C Wu, A. Chung, and J. McDonald A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly J. Cell Sci., January 6, 1995; 108(6): 2511 - 2523. [Abstract] [PDF]
|
|
|
|
|
|
R Winklbauer and C Stoltz Fibronectin fibril growth in the extracellular matrix of the Xenopus embryo J. Cell Sci., January 4, 1995; 108(4): 1575 - 1586. [Abstract] [PDF]
|
|
|
|
|
|
K Ichihara-Tanaka, K Titani, and K Sekiguchi Role of the carboxyl-terminal Fib2 domain in fibronectin matrix assembly J. Cell Sci., January 3, 1995; 108(3): 907 - 915. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Whittaker and D. DeSimone Integrin alpha subunit mRNAs are differentially expressed in early Xenopus embryos Development, January 4, 1993; 117(4): 1239 - 1249. [Abstract] [PDF]
|
|
|
|
|
|
B. R. Tomasini-Johansson, N. R. Kaufman, M. G. Ensenberger, V. Ozeri, E. Hanski, and D. F. Mosher A 49-Residue Peptide from Adhesin F1 of Streptococcus pyogenes Inhibits Fibronectin Matrix Assembly J. Biol. Chem., June 22, 2001; 276(26): 23430 - 23439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
