| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 262, Issue 9, 4104-4108, 03, 1987
RU Simpson, T Hsu, DA Begley, BS Mitchell and BN Alizadeh
Exposure of HL-60 promyelocytic leukemia cells to calcitriol results in a decrease in steady-state levels of c-myc mRNA and induces cellular differentiation. We have asked whether calcitriol has a direct effect on the transcription of the c-myc gene. 1,25-Dihydroxyvitamin D3 (1,25- (OH)2D3) decreased RNA elongation in a nuclear run-off transcription assay by 4 h after treatment. In the continuous presence of 1,25- (OH)2D3, HL-60 cell transcription of c-myc was decreased by 38% at 4 h and was abolished by 48 h. In contrast, the transcription of beta-actin was not affected by 1,25-(OH)2D3 treatment. The rate of transcription of c-myc and beta-actin was proportional to the number of nuclei and to time. Furthermore, specific hybridization of c-myc and beta-actin RNA was a linear function of RNA input. After a 48-h treatment, the c- myc/beta-actin ratio was decreased by 80-100% at [32P]RNA inputs ranging from 2 to 20 X 10(6) cpm/ml. These data temporally correlate inhibition of c-myc transcription with decreases in the steady-state levels of c-myc mRNA as assessed by Northern blot analysis. We conclude that the effect of 1,25-(OH)2D3 on c-myc expression occurs at the transcriptional level.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. A. LAMPRECHT and M. LIPKIN Cellular Mechanisms of Calcium and Vitamin D in the Inhibition of Colorectal Carcinogenesis Ann. N.Y. Acad. Sci., December 1, 2001; 952(1): 73 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Jensen, M. W. Madsen, J. Lukas, L. Binderup, and J. Bartek Inhibitory Effects of 1{alpha},25-Dihydroxyvitamin D3 on the G1-S Phase-Controlling Machinery Mol. Endocrinol., August 1, 2001; 15(8): 1370 - 1380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Agadir, G. Lazzaro, Y. Zheng, X.-K. Zhang, and R. Mehta Resistance of HBL100 human breast epithelial cells to vitamin D action Carcinogenesis, April 1, 1999; 20(4): 577 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Pan and R. U. Simpson c-myc Intron Element-binding Proteins Are Required for 1,25-Dihydroxyvitamin D3 Regulation of c-myc during HL-60 Cell Differentiation and the Involvement of HOXB4 J. Biol. Chem., March 26, 1999; 274(13): 8437 - 8444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. U. Simpson, T. D. O'Connell, Q. Pan, J. Newhouse, and M. J. Somerman Antisense Oligonucleotides Targeted against Protein Kinase Cbeta and Cbeta II Block 1,25-(OH)2D3-induced Differentiation J. Biol. Chem., July 31, 1998; 273(31): 19587 - 19591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Kaptein, C.-K. E. Lin, C. L. Wang, T. T. Nguyen, C. I. Kalunta, E. Park, F.-S. Chen, and P. M. Lad Anti-IgM-mediated Regulation of c-myc and Its Possible Relationship to Apoptosis J. Biol. Chem., August 2, 1996; 271(31): 18875 - 18884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Kwak, D. A. Larochelle, C. Beaumont, S. V. Torti, and F. M. Torti Role for NF-[IMAGE]B in the Regulation of Ferritin H by Tumor Necrosis Factor-[IMAGE] J. Biol. Chem., June 25, 1995; 270(25): 15285 - 15293. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
