HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klig, L. S. Articles by Yanofsky, C. Search for Related Content PubMed PubMed Citation Articles by Klig, L. S. Articles by Yanofsky, C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 1, 243-246, Jan, 1988

Increased binding of operator DNA by trp superrepressor EK49

LS Klig and C Yanofsky
Department of Biological Sciences, Stanford University, California 94305-5020.

The mechanism of superrepression by the mutant trp repressor EK49 was examined. This superrepressor has a glutamic acid-to-lysine change at residue 49. The purified EK49 trp repressor was found to have a 10-fold higher affinity than wild type repressor for trp operator DNA. This increased affinity was shown to be due to a decrease in dissociation rate. The binding of trp operator DNA by EK49 trp repressor in the filter binding assay was more sensitive to high salt concentrations than binding by wild type repressor.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. D. Finucane and O. Jardetzky Surface plasmon resonance studies of wild-type and AV77 tryptophan repressor resolve ambiguities in super-repressor activity Protein Sci., August 1, 2003; 12(8): 1613 - 1620. [Abstract] [Full Text] [PDF]

				A. M. Grunden, W. T. Self, M. Villain, J. E. Blalock, and K. T. Shanmugam An Analysis of the Binding of Repressor Protein ModE to modABCD (Molybdate Transport) Operator/Promoter DNA of Escherichia coli J. Biol. Chem., August 20, 1999; 274(34): 24308 - 24315. [Abstract] [Full Text] [PDF]

				M. N. Alekshun and S. B. Levy Characterization of MarR Superrepressor Mutants J. Bacteriol., May 15, 1999; 181(10): 3303 - 3306. [Abstract] [Full Text]