| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 14, 6703-6708, 05, 1988
FG Hamel, BI Posner, JJ Bergeron, BH Frank and WC Duckworth
Rats were injected with [125I]iodoinsulin labeled at either the A14 or B26
tyrosine, and the animals were killed and livers subcellularly fractionated
to yield light (early or neutral) endosomes and heavy (late or acidic)
endosomes. 125I-Labeled material was extracted from endosomes and analyzed
by Sephadex G-50 filtration and high performance liquid chromatography
(HPLC). Radiolabeled material in both types of endosomes is comprised of
high molecular weight, insulin-sized, and low molecular weight components,
with B chain-labeled small molecular weight material in two peaks, one
corresponding to iodotyrosine and one to small peptides (Mr less than
1500). As compared with A chain label, however, less of the B chain
material appears in the degradation components (both high and low molecular
weight fractions) suggesting that a fragment of B chain containing the B26
residue is lost from the endosomes. Analysis on HPLC shows that significant
amounts of the insulin-sized and high molecular weight material have
proteolytic cleavage(s) in the B chain with an intact A chain. The B
chain-derived labeled peptides elute from HPLC identically with products
generated by insulin protease. These results therefore show substantial
insulin degradation occurring in light endosomes prior to endosomal
acidification and to receptor dissociation, suggesting receptor-bound
insulin is a substrate for insulin protease.
Isolation of insulin degradation products from endosomes derived from intact rat liver
Veterans Administration Medical Center, Omaha, Nebraska 68105.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Balbis, G. Baquiran, V. Dumas, and B. I. Posner Effect of Inhibiting Vacuolar Acidification on Insulin Signaling in Hepatocytes J. Biol. Chem., March 26, 2004; 279(13): 12777 - 12785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. G. Hamel, J. L. Upward, and R. G. Bennett In Vitro Inhibition of Insulin-Degrading Enzyme by Long-Chain Fatty Acids and Their Coenzyme A Thioesters Endocrinology, June 1, 2003; 144(6): 2404 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Authier, M. Metioui, S. Fabrega, M. Kouach, and G. Briand Endosomal Proteolysis of Internalized Insulin at the C-terminal Region of the B Chain by Cathepsin D J. Biol. Chem., March 8, 2002; 277(11): 9437 - 9446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. C. Duckworth, R. G. Bennett, and F. G. Hamel Insulin Degradation: Progress and Potential Endocr. Rev., October 1, 1998; 19(5): 608 - 624. [Abstract] [Full Text]
|
|
|
|
|
|
J.-O. Contreres, R. Faure, G. Baquiran, J. J. Bergeron, and B. I. Posner ATP-dependent Desensitization of Insulin Binding and Tyrosine Kinase Activity of the Insulin Receptor Kinase. THE ROLE OF ENDOSOMAL ACIDIFICATION J. Biol. Chem., August 21, 1998; 273(34): 22007 - 22013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Chesneau, R. K. Perlman, W. Li, G.-A. Keller, and M. R. Rosner Insulin-Degrading Enzyme Does Not Require Peroxisomal Localization for Insulin Degradation Endocrinology, August 1, 1997; 138(8): 3444 - 3451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tjelle, A Brech, L. Juvet, G Griffiths, and T Berg Isolation and characterization of early endosomes, late endosomes and terminal lysosomes: their role in protein degradation J. Cell Sci., January 12, 1996; 109(12): 2905 - 2914. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
