J. Biol. Chem., Vol. 263, Issue 15, 6960-6963, 05, 1988
Inhibition of the RNA polymerase-catalyzed synthesis of RNA by marcellomycin. Preferential interference of the inhibitor with the stabilization of the ternary promoter-RNA polymerase-nascent RNA complex
T Kriebardis and S Aktipis
Department of Biochemistry and Biophysics, Stritch School of Medicine, Loyola University, Maywood, Illinois 60153.
Marcellomycin is a strong inhibitor of the Escherichia coli RNA
polymerase-catalyzed synthesis of RNA from the strong A promoters of
bacteriophage T7 DNA. Marcellomycin inhibits preferentially the last phase
of transcription initiation. During this phase a stabilized ternary complex
is formed consisting of RNA polymerase, DNA template, and a nascent RNA
oligonucleotide about 11 nucleotides long, resulting from the extension of
the RNA dinucleotide component of the corresponding early ternary complex.
Marcellomycin is also responsible for minor inhibition of the formation of
the open binary RNA polymerase- template complex, which serves as the
precursor of the ternary complex. These findings suggest that marcellomycin
may be a potentially useful tool in the study of the late stages of
transcription initiation. The present findings may also contribute to a
better overall understanding of the mode of drug action at the level of
individual genes.
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