HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geiger, T. Articles by Heinrich, P. C. Search for Related Content PubMed PubMed Citation Articles by Geiger, T. Articles by Heinrich, P. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 15, 7141-7146, 05, 1988

Induction of alpha 1-acid glycoprotein by recombinant human interleukin- 1 in rat hepatoma cells

T Geiger, T Andus, J Klapproth, H Northoff and PC Heinrich
Biochemisches Institut, Universitat Freiburg, Federal Republic of Germany.

The induction of alpha 1-acid glycoprotein mRNA by recombinant murine interleukin-1, recombinant human interleukin-1 alpha, and recombinant human interleukin-1 beta has been studied in the rat hepatoma cell line Fao. Whereas the stimulatory capacities of recombinant human interleukin-1 alpha and recombinant murine interleukin-1 were almost identical, the concentrations of recombinant human interleukin-1 beta needed for half-maximal induction of alpha 1-acid glycoprotein mRNA were lower by three orders of magnitude. A 60-fold increase in alpha 1- acid glycoprotein mRNA levels was observed 18 h after the addition of recombinant interleukin-1 beta. In parallel albumin mRNA levels decreased to about 30%. The alpha 1-acid glycoprotein mRNA induction was strictly dependent on the presence of dexamethasone. For a full stimulation dexamethasone concentrations of greater than 10(-7) M were needed, whereas concentrations of less than 10(-12) M were ineffective. The increase in alpha 1-acid glycoprotein mRNA after recombinant human interleukin-1 beta was followed by a 36-fold stimulation in alpha 1- acid glycoprotein synthesis and secretion. When protein synthesis was blocked by either cycloheximide, puromycin, or emetine, the induction of alpha 1-acid glycoprotein mRNA by recombinant human interleukin-1 beta was impaired suggesting the involvement of a short-lived protein in the induction of alpha 1-acid glycoprotein mRNA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Fournier, N. Bouach, C. Delafosse, B. Crestani, and M. Aubier Inducible Expression and Regulation of the {alpha}1-Acid Glycoprotein Gene by Alveolar Macrophages: Prostaglandin E2 and Cyclic AMP Act as New Positive Stimuli J. Immunol., September 1, 1999; 163(5): 2883 - 2890. [Abstract] [Full Text] [PDF]

				B. Crestani, C. Rolland, B. Lardeux, T. Fournier, D. Bernuau, C. Pous, C. Vissuzaine, L. Li, and M. Aubier Inducible Expression of the {alpha}1-Acid Glycoprotein by Rat and Human Type II Alveolar Epithelial Cells J. Immunol., May 1, 1998; 160(9): 4596 - 4605. [Abstract] [Full Text] [PDF]